Elastase from Porcine Pancreas
Related Categories: Application rate, Biochemicals and reagents, Enzymes, Inhibitors and substrates, Isolation of proteins and nucleic acids
Quality level: 100
Form: lyophilized
Specific activity: ≥25 units / mg protein
Storage condition:
OK to freeze
Dried
Storage conditions: -20C
General description
Elastase is native to the porcine pancreas. Serine protease that catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin, and of various synthetic substrates containing aspartic acid, phenylalanine, or tyrosine. Inhibited by DFP, elastin, and α2-macroglobulin.
Elastase is native to the porcine pancreas. Catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin, and of various synthetic substrates containing aspartic acid, glutamic acid, phenylalanine, or tyrosine. Preferably it cleaves peptide bonds at the carbonyl end of amino acid residues with small hydrophobic side chains, such as glycine, valine, leucine, isoleucine, and particularly alanine. Inhibited by DFP, elastin, and α2-macroglobulin. It has an optimal pH of 7.8-8.5; pI = 9.5.
Packaging
1000, 250 u in plastic ampoule
Warning
Toxicity: Standard handling (A)
Definition of unit
One unit is defined as the amount of enzyme that will hydrolyze 1.0 mole of Suc-Ala-Ala-Pro-Abu-PNA (catalog # 324699) per minute at 25 ° C, pH 8.0.
Physical form
Lyophilized from 50 mM trehalose, 1 mM acetic acid.
Reconstitution
After reconstitution, elastase can be stored at 4 ° C at pH 6.0 for long-term use. If incubated at room temperature at or near its optimum pH, elastase rapidly autolyzes to a mixture of peptides. Stock solutions are stable for up to 2 months at 4 ° C, pH 6.0.