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Elastase from Porcine Pancreas

Related Categories: Application rate, Biochemicals and reagents, Enzymes, Inhibitors and substrates, Isolation of proteins and nucleic acids

Quality level: 100

Form: lyophilized

Specific activity: ≥25 units / mg protein

Storage condition:

OK to freeze
Dried

Storage conditions: -20C

General description

Elastase is native to the porcine pancreas. Serine protease that catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin, and of various synthetic substrates containing aspartic acid, phenylalanine, or tyrosine. Inhibited by DFP, elastin, and α2-macroglobulin.

Elastase is native to the porcine pancreas. Catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin, and of various synthetic substrates containing aspartic acid, glutamic acid, phenylalanine, or tyrosine. Preferably it cleaves peptide bonds at the carbonyl end of amino acid residues with small hydrophobic side chains, such as glycine, valine, leucine, isoleucine, and particularly alanine. Inhibited by DFP, elastin, and α2-macroglobulin. It has an optimal pH of 7.8-8.5; pI = 9.5.

Packaging

1000, 250 u in plastic ampoule

Warning

Toxicity: Standard handling (A)

Definition of unit

One unit is defined as the amount of enzyme that will hydrolyze 1.0 mole of Suc-Ala-Ala-Pro-Abu-PNA (catalog # 324699) per minute at 25 ° C, pH 8.0.

Physical form

Lyophilized from 50 mM trehalose, 1 mM acetic acid.

Reconstitution

After reconstitution, elastase can be stored at 4 ° C at pH 6.0 for long-term use. If incubated at room temperature at or near its optimum pH, elastase rapidly autolyzes to a mixture of peptides. Stock solutions are stable for up to 2 months at 4 ° C, pH 6.0.

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