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Cell Counting Kit-8 (CCK-8)

Cell Counting Kit-8 (CCK-8) permits helpful tests utilizing WST-8 (2-(2-methoxy-4-nitrophenyl)- 3-(4-nitrophenyl)- 5-(2,4-disulfophenyl)- 2H-tetrazolium, monosodium salt), which delivers a water-dissolvable formazan color upon bioreduction within the sight of an electron transporter, 1-Methoxy PMS. CCK-8 arrangement is added straightforwardly to the cells, no pre-blending of parts is required. WST-8 is bioreduced by cell dehydrogenases to an orange formazan item that is solvent in tissue culture medium.

How much formazan created is straightforwardly corresponding to the quantity of living cells. Since the CCK-8 arrangement is entirely steady and it has little cytotoxicity, a more extended brooding, like 24 to 48 hours, is conceivable.
Cell Counting Kit-8 permits touchy colorimetric examines for the assurance of the quantity of reasonable cells in the multiplication and cytotoxicity tests. The recognition responsiveness is higher than some other tetrazolium salts like MTT, XTT or MTS.

Cell Counting Kit 8, ccK-8 for short, is a profoundly delicate and non-radioactive colorimetric identification strategy used to decide the quantity of living cells in Cell multiplication or harmfulness tests.

Cck-8 is an update result of MTT. Cck-8 arrangement can be straightforwardly added to cell tests without prepreparation of different parts, fast recognition and extremely low poisonousness. Cck-8 depends on the water-dissolvable tetrazole salt WST-8.

This is the secret: WST-8, within the sight of an electron-coupled reagent, can be decreased by a chemical called dehydrogenase in the mitochondria to create an orange messy color. Nail dark breaks down in tissue media with respect to the quantity of living cells. By colorimetry, the quantity of living cells can be progressively evaluated to recognize cell multiplication or medication poisonousness. This unit is ordinarily used to identify cycle and apoptosis of connected or suspended cells. On account of tissue, the tissue should be processed into a solitary cell state before it tends to be tried.

The TC20 mechanized cell counter includes mammalian cells in a single basic advance utilizing its inventive auto-center innovation and complex cell counting calculation to create exact cell includes in under 30 seconds. Endless supply of an including slide, the TC20 cell counter quickly gives an absolute cell count (no matter what trypan blue staining) and surveys cell practicality by means of trypan blue rejection..

Key Features and Benefits

  • Viable with an expansive scope of cell sizes and types – counts cell lines, essential cells (from tissue or blood), and undifferentiated organisms
  • Creative auto-center innovation – eliminates the variety related with manual centering and prompts exact cell includes in 30 seconds
  • Cell size entryways – client chooses a populace of interest in complex examples, like essential cells, or lets the cell counting calculation accomplish practically everything
  • Cell practicality – investigates cells precisely utilizing multifocal plane examination
  • Simple to file and investigate – accumulates to 100 includes in the installed memory for access any time, or utilize the discretionary TC20 information analyzer programming on your PC to additionally break down sent out cell pictures
  • Getting every one of the information you really want about your cell societies is quick and simple; the TC20 cell counter and expendable counting slides dispense with the requirement for dreary arrangement, cleaning, or upkeep.
  • The TC20 cell counter is straightforward and instinctive to utilize – figure out how to utilize the TC20 mechanized cell counter in the presentation video or step through a virtual examination drive.

Come by Accurate and Reproducible Results

The TC20 robotized cell counter purposes microscopy with auto-center that examines various central planes to distinguish the best plane. Without requiring any client input, the refined cell counting calculation utilizes the picture obtained from the best central plane to distinguish cells and prohibit trash, consequently working out the complete cell count.

Cell Counting Kit-8
DLSM0248 DL Develop 500 Assays Ask for price

Cell Counting Kit-8
CSK1175 Alphabioregen 5ml 0.1 EUR

Cell Counting Kit-8
HY-K0301 MedChemExpress 120 mL (12000 T) 1362 EUR

Cell Counting Kit-8
MBS8305412-100Assays MyBiosource 100Assays 275 EUR

Cell Counting Kit-8
MBS8305412-500Assays MyBiosource 500Assays 365 EUR

Cell Counting Kit-8
MBS8305412-5x500Assays MyBiosource 5x500Assays 1580 EUR

Cell Counting Kit-8
RDSM248 Reddot Biotech 500 Assays 245 EUR

Cell Counting Kit-8 (CCK-8)
B34302 Bimake 5 mL (500 ractions) 156 EUR

Cell Counting Kit-8 (CCK-8)
B34304 Bimake 25 mL (2500 ractions) 378 EUR

Cell Counting Kit-8 (CCK-8)
AR1160 BosterBio 5 mL (for 500 assays, 10μL per well) 80 EUR

Cell Counting Kit-8 (CCK-8)
C0005-10mg TargetMol Chemicals 10mg Ask for price

Cell Counting Kit-8 (CCK-8)
C0005-1g TargetMol Chemicals 1g Ask for price

Cell Counting Kit-8 (CCK-8)
C0005-1mg TargetMol Chemicals 1mg Ask for price

Cell Counting Kit-8 (CCK-8)
C0005-50mg TargetMol Chemicals 50mg Ask for price

Cell Counting Kit-8 (CCK-8)
C0005-5mg TargetMol Chemicals 5mg Ask for price

Cell Counting Kit-8 (CCK-8)
K1018-1 ApexBio 1x1 ml (100 tests) 40 EUR

Cell Counting Kit-8 (CCK-8)
K1018-10 ApexBio 2x5 ml (1000 tests) 80 EUR

Cell Counting Kit-8 (CCK-8)
K1018-100 ApexBio 20x5 ml (10000 tests) 576 EUR

Cell Counting Kit-8 (CCK-8)
K1018-30 ApexBio 6x5 ml (3000 tests) 192 EUR

The auto-center prompts profoundly reproducible cell counts with decreased client to-client inconstancy contrasted with a hemocytometer and cell counters with manual concentration. Utilizing auto-center rather than emotional manual centering is particularly significant while surveying cell reasonability on the grounds that an erroneously chosen central plane will prompt wrong outcomes.

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lyo NZYSupreme qPCR Probe Master Mix
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lyo NZYSupreme qPCR Probe Master Mix

Description: Lyo NZYSupreme qPCR Probe Master Mix (2x) is an optimized and highly efficient freeze-dried reaction mixture developed for realtime PCR. This master mix was engineered with a dual hot-start enzyme control mechanism to provide the highest detection sensitivity. In addition, the latest developments in PCR enhancers have been incorporated in the Lyo NZYSupreme qPCR Probe Master Mix, including buffer chemistry and incorporation of highly robust engineered enzymes. This master mix does not contain ROX and it was specifically developed for probe-detection technology, including molecular beacons. For qPCR instruments that require ROX reference dye, please add ROX (Cat. No. MB406) according to the table presented in the section “ROX reference dye”. Lyo NZYSupreme qPCR Probe Master Mix (2x) is provided as a simple-to-use, stabilized 2x reaction mixture that includes all components for quantitative PCR, except sample DNA, primers, probe and water.

Features:
– Eco-friendly room temperature shipment
– Stable at room temperature for 1 month
– Dual hot-start mode
– Ultra-sensitive: detects low-copy number targets
– Batch-to-batch reproducibility
– Intra-batch reproducibility
– Simple and reproducible
– Compatible with multiple real-time platforms

Components:
– Lyo NZYSupreme qPCR Probe Master Mix (2x)
– qPCR master mix reconstitution buffer

Applications:
– Real-time qPCR
– Two-step RT-qPCR
– Developed for probe-detection technology

New Lyo qPCR Probe Master Mix (2x)

Lyo NZYSupreme qPCR Probe Master Mix (2x)

Description: Lyo NZYSupreme qPCR Probe Master Mix (2x) is an optimized and highly efficient freeze-dried reaction mixture developed for realtime PCR. This master mix was engineered with a dual hot-start enzyme control mechanism to provide the highest detection sensitivity. In addition, the latest developments in PCR enhancers have been incorporated in the Lyo NZYSupreme qPCR Probe Master Mix, including buffer chemistry and incorporation of highly robust engineered enzymes. This master mix does not contain ROX and it was specifically developed for probe-detection technology, including molecular beacons. For qPCR instruments that require ROX reference dye, please add ROX (Cat. No. MB406) according to the table presented in the section “ROX reference dye”. Lyo NZYSupreme qPCR Probe Master Mix (2x) is provided as a simple-to-use, stabilized 2x reaction mixture that includes all components for quantitative PCR, except sample DNA, primers, probe and water.

lyo NZYSupreme qPCR Probe Master Mix
lyo NZYSupreme qPCR Probe Master Mix

Features:
– Eco-friendly room temperature shipment
– Stable at room temperature for 1 month
– Dual hot-start mode
– Ultra-sensitive: detects low-copy number targets
– Batch-to-batch reproducibility
– Intra-batch reproducibility
– Simple and reproducible
– Compatible with multiple real-time platforms

Components:
– Lyo NZYSupreme qPCR Probe Master Mix (2x)
– qPCR master mix reconstitution buffer

Apex qPCR Probe Master Mix
42-118P 400 x 25µl Reactions/Unit
EUR 341.44
Description: 2X, Low ROX, 5ml

Apex qPCR Probe Master Mix
42-119P 4000 x 25µl Reactions/Unit
EUR 2926.74
Description: 2X, Low ROX, 50ml

Apex qPCR Probe Master Mix
42-120P 400 x 25µl Reactions/Unit
EUR 341.44
Description: 2X, High ROX, 5ml

Apex qPCR Probe Master Mix
42-121P 4000 x 25µl Reactions/Unit
EUR 2926.74
Description: 2X, High ROX, 50ml

AceQ qPCR Probe Master Mix
Q112-02 500 rxn (20 μl/rxn)
EUR 120.55

AceQ qPCR Probe Master Mix
Q112-03 2500 rxn (20 μl/rxn)
EUR 543.5

Probe qPCR Master Mix (2x)
E0420-01 100reactions
EUR 55.59
Description: For quantitative real-time PCR and two-step real-time RT-PCR Using sequence-specific probes

Probe qPCR Master Mix (2x)
E0420-02 200reactions
EUR 106.82
Description: For quantitative real-time PCR and two-step real-time RT-PCR Using sequence-specific probes

Probe qPCR Master Mix (2x)
E0420-03 1000reactions
EUR 467.61
Description: For quantitative real-time PCR and two-step real-time RT-PCR Using sequence-specific probes

Entrans 2X qPCR Probe Master Mix
RK21208 40 RXN
EUR 185.3

MethyLight 2X qPCR Probe Master Mix
RK21240 1mL
EUR 209.83

Entrans 2X qPCR Probe Master Mix V2
RK21212 50RXN
EUR 40.88

Fast Probe qPCR Master Mix (2x)
E0422-01 100reactions
EUR 55.59
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR Using sequence-specific probes

Fast Probe qPCR Master Mix (2x)
E0422-02 200reactions
EUR 106.82
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR Using sequence-specific probes

Fast Probe qPCR Master Mix (2x)
E0422-03 1000reactions
EUR 467.61
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR Using sequence-specific probes

BioEasy Multiplex qPCR Probe Master Mix II
TRI-B71M1 500T
EUR 619.92

BioEasy Multiplex qPCR Probe Master Mix II
TRI-B71S1 100T
EUR 138.6

Entrans 2X qPCR Probe Master Mix with UDG
RK21222 50RXN
EUR 51.78

2X qPCR Probe Master Mix with Separate ROX
MBS308018-1000Reactions 1000Reactions
EUR 1150

2X qPCR Probe Master Mix with Separate ROX
MBS308018-100Reactions 100Reactions
EUR 210

2X qPCR Probe Master Mix with Separate ROX
MBS308018-2500Reactions 2500Reactions
EUR 5350

2X qPCR Probe Master Mix with Separate ROX
MBS308018-500Reactions 500Reactions
EUR 405

HotStart™ 2X Probe qPCR Master Mix
K1541-25 25ml
EUR 437.6
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ 2X Probe qPCR Master Mix
K1541-5 5ml
EUR 123.2
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ 2X Probe qPCR Master Mix
K1541-50 50ml
EUR 866.4
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

igScript™ Probe-Based qPCR master mix
4233 500
EUR 172
Description: IgScript™ probe-based qPCR 2x master mix contains IgScript™ Taq DNA polymerase, MgCl2, dNTPs, stabilizers, enhancers and low ROX reference dye with standard buffer providing improved qPCR efficiency, wider dynamic range, superior sensitivity and specificity. IgScript™ qPCR 2x master mix is a ready-to-use cocktail containing all components except primers, probe and template, for the amplification and detection of DNA in qPCR. This 2x master mix requires minimal handling during reaction setup and offer consistent and robust qPCR reactions. Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase (1, 2) and a 5´→3´ exonuclease activity (3, 4). The amplification step features a high quality Taq DNA Polymerase which offers robust, reliable and better amplification.Product IncludesigScript™ Probe-Based qPCR master mixApplicationsGene expression data validation.MultiplexingMutation detectionPathogen and viral detectionGenetically modified organisms (GMO) characterization and Genetic profiling

igScript™ Probe-Based qPCR master mix
4235 1000
EUR 276
Description: IgScript™ probe-based qPCR 2x master mix contains IgScript™ Taq DNA polymerase, MgCl2, dNTPs, stabilizers, enhancers and low ROX reference dye with standard buffer providing improved qPCR efficiency, wider dynamic range, superior sensitivity and specificity. IgScript™ qPCR 2x master mix is a ready-to-use cocktail containing all components except primers, probe and template, for the amplification and detection of DNA in qPCR. This 2x master mix requires minimal handling during reaction setup and offer consistent and robust qPCR reactions. Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase (1, 2) and a 5´→3´ exonuclease activity (3, 4). The amplification step features a high quality Taq DNA Polymerase which offers robust, reliable and better amplification.Product IncludesigScript™ Probe-Based qPCR master mixApplicationsGene expression data validation.MultiplexingMutation detectionPathogen and viral detectionGenetically modified organisms (GMO) characterization and Genetic profiling

igScript™ Probe-Based qPCR master mix
4237 2500
EUR 559.2
Description: IgScript™ probe-based qPCR 2x master mix contains IgScript™ Taq DNA polymerase, MgCl2, dNTPs, stabilizers, enhancers and low ROX reference dye with standard buffer providing improved qPCR efficiency, wider dynamic range, superior sensitivity and specificity. IgScript™ qPCR 2x master mix is a ready-to-use cocktail containing all components except primers, probe and template, for the amplification and detection of DNA in qPCR. This 2x master mix requires minimal handling during reaction setup and offer consistent and robust qPCR reactions. Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase (1, 2) and a 5´→3´ exonuclease activity (3, 4). The amplification step features a high quality Taq DNA Polymerase which offers robust, reliable and better amplification.Product IncludesigScript™ Probe-Based qPCR master mixApplicationsGene expression data validation.MultiplexingMutation detectionPathogen and viral detectionGenetically modified organisms (GMO) characterization and Genetic profiling

 

 

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t.spot covid
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t.spot covid

HIGHLIGHTS

  • •
    TSPOT.COVID is an ELISpot interferon gamma-release assay for SARS-CoV-2
  • •
    TSPOT.COVID identifies a T cell response to SARS-CoV-2 spike S1 and N peptides
  • •
    2–8 weeks post SARS-CoV-2 diagnosis TSPOT.COVID detected 98% of infections
  • •
    In comparison, immunoglobulin G (IgG) serology detected 83% of infections in the same period
  • •
    Cellular immune response activated sooner and lasted longer than antibodies

Abstract

Objective

To evaluate the performance of the T-SPOT.COVID test for identifying SARS-CoV-2-responsive T-cells in participants with SARS-CoV-2 infection.

Methods

The T-SPOT.COVID test uses ELISpot interferon-gamma release assay (IGRA) methodology to measure T cell responses to SARS-CoV-2 spike S1 and nucleocapsid peptides. T-SPOT.COVID and anti-N immunoglobulin (Ig) G serology tests were performed on blood from 186 patients with nucleic acid amplification test (NAAT)-confirmed-SARS-CoV-2 infection and 100 control group participants.

Results

In the 2–8 weeks after NAAT-diagnosed SARS-CoV-2 infection, the T-SPOT.COVID test detected 98.4% (63 of 64) of infected participants, while anti-N IgG serology detected 82.8%. In the first 2 weeks after diagnosis, during adaptive immune response activation, there were less reactive T-SPOT.COVID responses (75.7%, 28 of 37 infected participants) and many less seropositive responses (32.4%). Response numbers tapered after 8 weeks; however, T-SPOT.COVID test continued to detect most participants with confirmed infection (83.6%, 56 of 67) and continued to out-perform serology (52.2%). T-SPOT.COVID response due to cross-reactive T cells was ruled out by demonstrating that, of 44 control group participants with T cells responsive to 4 human common cold coronavirus peptides, only 1 was T-SPOT.COVID reactive.

Conclusion

The T-SPOT.COVID test performed well in detecting SARS-CoV-2-sensitized T-cells over many months

Introduction

Long-term protection from infectious agents, such as the SARS-CoV-2 virus, is mediated by T cells and antibody-mediated immunity of the adaptive immune system (

Sette and Crotty, 2021

). The T-SPOT.COVID test was developed to identify the presence of SARS-CoV-2-responsive T cells.

T cells contribute to the understanding of SARS-CoV-2 infections in many ways. T cells can identify past SARS-CoV-2 infections at a time when PCR tests would be negative and antibodies levels may be waning (

Dan et al., 2021

;

Gudbjartsson et al., 2020

;

Poland et al., 2020

). T cells can provide immune memory lasting for months (

Dan et al., 2021

) and perhaps years, as suggested by the discovery of T cells to the SARS-CoV-1 coronavirus 17 years after infection (

Le Bert et al., 2020

). T cells may act independently of antibodies to control a SARS-CoV-2 infection, as shown by the recovery of COVID-19 patients who lack detectable antibodies but have SARS-CoV-2-responsive T cells (

Gallais et al., 2021

;

Sekine et al., 2020

). T cells also show reactivity to numerous SARS-CoV-2 epitopes, so have the potential to protect against many SARS-CoV-2 variants (

Grifoni et al., 2020

;

Tarke et al., 2021

). T cell-based assays can probe the longevity of an immune response following a SARS-CoV-2 infection or vaccination (

Goletti et al., 2021

;

Liu et al., 2021

;

Reynolds et al., 2021

). These various roles suggest that a T cell assay can be a key contributor to SARS-CoV-2 investigations.

The T-SPOT.COVID test, an enzyme-linked immunospot (ELISpot) assay, identifies T cells in peripheral blood that release interferon-gamma (IFN-γ) in response to stimulation with SARS-CoV-2 peptides. The T-SPOT.COVID test builds on the T-SPOT platform (Oxford Immunotec) used worldwide for tuberculosis and cytomegalovirus testing and the research version, the T-SPOT Discovery SARS-CoV-2 test (

Liu et al., 2021

;

t.spot covid
t.spot covid
Wyllie et al., 2021

). The T-SPOT.COVID ELISpot methodology is performed in many laboratories and offers a standardized comparison of T cell immunity among participants. In addition, ELISpot assays normalize the number of peripheral blood mononuclear cells (PBMCs), thus maintaining test effectiveness in participants with lymphopenia, a commonly reported condition in many COVID-19 patients (

Altmann and Boyton, 2020

) and immunosuppressed people.

The objective of this study was to evaluate the ability of the T-SPOT.COVID test to detect T cell responses in participants with or without a history of SARS-CoV-2 infection and to compare the T-SPOT.COVID test results with anti-N immunoglobulin (Ig)G serology results in the first several months after infection.

Materials and Methods

2.1 Participant recruitment

Participants for this single-center, cross-sectional study were recruited from patients who had attended the outpatient Primacare medical center in Fall River, Massachusetts, USA, between November 30, 2020, and March 24, 2021, a time of high demand for COVID-19 testing. Among other healthcare services, Primacare provided COVID-19 testing to anyone wanting or required to be tested. The New England Center for Clinical Research (NECCR) invited participants to join the study if they had received a positive SARS-CoV-2 nucleic acid amplification test (NAAT) at Primacare or if NECCR deemed them to be at low risk of SARS-CoV-2 infection. As this study was run independently from the participants’ healthcare providers, clinical data such as chest x-rays and hospitalizations records were not obtained. Informed consent and study approval were obtained from the Advarra institutional review board by NECCR at Primacare.
Confirmed-infection group: A NAAT, which detects the presence of the SARS-CoV-2 virus, was used to identify people infected with SARS-CoV-2 at the time of testing (

Rai et al., 2021

). Participants in the confirmed-infection group were recruited from asymptomatic and symptomatic patients who had had a positive SARS-CoV-2 NAAT result within the past 9 months. The date of the first positive NAAT result was considered the date of diagnosis of SARS-CoV-2 infection. Blood was drawn for Abbott SARS-CoV-2 chemiluminescent microparticle immunoassay (CMIA) anti-N IgG serology and T-SPOT.COVID tests between 0 to 249 days after diagnosis.

The analysis of responses was divided into 3 time periods: 0 to 2 weeks after diagnosis (0 to 14 days); 2+ to 8 weeks after diagnosis (15 to 56 days); and 8+ weeks after diagnosis (57+ days).
Control group: Many SARS-CoV-2 studies use frozen pre-pandemic blood for control samples; however, the T-SPOT platform requires fresh blood to ensure consistent results. Therefore fresh blood was obtained from control group participants prospectively recruited from individuals with low risk of prior SARS-CoV-2 infection. Requirements for enrollment included no current or prior signs or symptoms of COVID-19, no known contact with a confirmed SARS-CoV-2-infected individual, no prior history of a positive SARS-CoV-2 NAAT, no SARS-CoV-2 vaccination, and no prior diagnosis with SARS-CoV-1 or Middle Eastern Respiratory Syndrome (MERS). In addition, the BIOHIT HealthCare SARS-CoV-2 lateral flow anti-N IgM/IgG serology test was performed at enrollment, and the 1 person with a positive BIOHIT result was not enrolled. Blood was drawn at enrollment for testing with T-SPOT.COVID and the Abbott CMIA anti-N IgG serology test and anyone with a positive serology result was excluded from the control group.

2.2 T-SPOT.COVID test

The T-SPOT.COVID test includes over 250 SARS-CoV-2 peptides (15-mer peptides overlapping by 11 amino acids) in 2 antigen peptide pools; one pool contains peptides from the spike S1 protein, including the receptor-binding domain, and the other contains peptides from the nucleocapsid protein.
Blood samples for the T-SPOT.COVID test were processed and analyzed according to the manufacturer’s instructions. Briefly, blood samples were drawn into lithium heparin tubes which were shipped overnight to Oxford Immunotec (Abingdon, UK) in temperature-controlled shipping boxes. Next, the T-Cell Xtend reagent (Oxford Immunotec) was added to the samples, and PBMCs were isolated by density gradient centrifugation, washed, counted, and 250 000 cells/well were plated into 4 wells of a 96-well plate.

COVID-19 variant pack
SB072 0.5
EUR 2325.81
Description: SARS-CoV-2 Spike (S) glycoprotein:Spike (S) glycoprotein corresponds to one of the leading targets for COVID-19 disease. Present on the surface of Sars-CoV-2 virus, Spike S protein in a class I fusion protein that allows the virus to enter host cells.Variants package available:Some proteins of Sars-CoV-2 are identified as leading targets for COVID-19 therapies. SB-PEPTIDE offers a special pack pre-made peptide libraries of Spike protein including peptides from COVID-19 variants: UK COVID-19 variant B.1.1.7, South Africa COVID-19 variant B.1.351, Brazil COVID-19 variant B.1.1.248. SB-PEPTIDE offers additional plate containing only peptides with Spike S protein mutation of COVID-19 B.1.1.7, B.1.3.5.1 and B.1.1.248 (cited below).Variants package and Spike (S) glycoprotein peptide library can be used for T-cell assays, immune monitoring, antigen specific T-cell stimulation, T-cell expansion and cellular immune response.

COVID-19 Spike Antigen
30-2018 1 mg
EUR 400
Description: COVID-19 Spike recombinant antigen

COVID-19 Spike Antigen
30-2019 1 mg
EUR 400
Description: COVID-19 Spike recombinant antigen

Moderna COVID-19 Vaccine
H7N7-319 1 vial
EUR 798.4
Description: Protein

SARS & COVID-19 coronavirus
3851 100 ug
EUR 330
Description: nucleoprotein

SARS & COVID-19 coronavirus
3861 100 ug
EUR 330
Description: nucleoprotein

SARS & COVID-19 coronavirus
3862 each
EUR 330
Description: nucleoprotein

SARS & COVID-19 coronavirus
3863 each
EUR 330
Description: nucleoprotein

SARS & COVID-19 coronavirus
3864 each
EUR 330
Description: nucleoprotein

COVID-19 Negative Control
MBS412771-05mL 0.5mL
EUR 100

COVID-19 Negative Control
MBS412771-5x05mL 5x0.5mL
EUR 305

Coronavirus COVID-19 Spike (COVID-19, SARS-CoV-2, 2019-nCoV) ELISA Kit
MBS7612502-10x96StripWells 10x96-Strip-Wells
EUR 3900

Coronavirus COVID-19 Spike (COVID-19, SARS-CoV-2, 2019-nCoV) ELISA Kit
MBS7612502-48StripWells 48-Strip-Wells
EUR 340

Coronavirus COVID-19 Spike (COVID-19, SARS-CoV-2, 2019-nCoV) ELISA Kit
MBS7612502-5x96StripWells 5x96-Strip-Wells
EUR 2045

Coronavirus COVID-19 Spike (COVID-19, SARS-CoV-2, 2019-nCoV) ELISA Kit
MBS7612502-96StripWells 96-Strip-Wells
EUR 455

COVID-19 S Protein / (GFP)– (6His) VLP COVID-19 S Protein / (GFP)– (6His) VLP
VLP001 1x108 VP/ml x 200ul
EUR 455
Description: COVID-19 Spike protein (S) Virus Like Particle, packaged with GFP genomic material. Particles were concentrated and provided in PBS solution

COVID-19 Nucleocapsid protein
30-2005 1 mg
EUR 700
Description: COVID-19 Nucleocapsid protein recombinant antigen

COVID-19 Nucleocapsid protein
30-2006 1 mg
EUR 700
Description: COVID-19 Nucleocapsid protein recombinant Antigen

COVID-19 Nucleocapsid antigen
30-2020 1 mg
EUR 400
Description: COVID-19 Nucleocapsid recombinant protein

COVID-19 Nucleocapsid antigen
30-2021 1 mg
EUR 400
Description: COVID-19 Nucleocapsid recombinant protein

Coronavirus (COVID-19) Antibody
MBS569951-1mg 1mg
EUR 655

Coronavirus (COVID-19) Antibody
MBS569951-5x1mg 5x1mg
EUR 2760

COVID-19 Nucleocapsid Protein
MBS669369-005mg 0.05mg
EUR 500

COVID-19 Nucleocapsid Protein
MBS669369-025mg 0.25mg
EUR 1010

COVID-19 Nucleocapsid Protein
MBS669369-5x025mg 5x0.25mg
EUR 4525

COVID-19 Nucleoprotein antibody
10-2890 1 mg
EUR 420
Description: Purified COVID-19 Nucleoprotein Antibody

COVID-19 Nucleoprotein antibody
10-2891 1 mg
EUR 420
Description: Purified COVID-19 Nucleoprotein Antibody

COVID-19 Nucleoprotein antibody
10-2894 1 mg
EUR 420
Description: Purified COVID-19 Nucleoprotein Antibody

COVID-19 Nucleoprotein antibody
MBS5316694-1mg 1mg
EUR 360

COVID-19 Nucleoprotein antibody
MBS5316694-5x1mg 5x1mg
EUR 1480

COVID-19 Nucleoprotein antibody
MBS5316695-1mg 1mg
EUR 360

COVID-19 Nucleoprotein antibody
MBS5316695-5x1mg 5x1mg
EUR 1480

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test for allergies airborne screen

Overview

During allergy skin tests, your skin is exposed to suspected allergy-causing substances (allergens) and is then observed for signs of an allergic reaction.

Along with your medical history, allergy tests may be able to confirm whether a particular substance you touch, breathe, or eat is causing symptoms.

 

Allergy skin tests are widely used to help diagnose allergic conditions, including:

  • Hay fever (allergic rhinitis)
  • Allergic asthma
  • Dermatitis (eczema)
  • Food allergies
  • Penicillin allergy
  • Bee venom allergy

Skin tests are generally safe for adults and children of all ages, including infants. In certain circumstances, though, skin tests aren’t recommended. Your doctor may advise against skin testing if you:

  • Have ever had a severe allergic reaction. You may be so sensitive to certain substances that even the tiny amounts used in skin tests could trigger a life-threatening reaction (anaphylaxis).
  • Take medications that could interfere with test results. These include antihistamines, many antidepressants and some heartburn medications. Your doctor may determine that it’s better for you to continue taking these medications than to temporarily discontinue them in preparation for a skin test.
  • Have certain skin conditions. If severe eczema or psoriasis affects large areas of skin on your arms and back — the usual testing sites — there may not be enough clear, uninvolved skin to do an effective test. Other skin conditions, such as dermatographism, can cause unreliable test results.

Blood tests (in vitro immunoglobulin E antibody tests) can be useful for those who shouldn’t or can’t undergo skin tests. Blood tests aren’t used for penicillin allergy.

In general, allergy skin tests are reliable for diagnosing allergies to airborne substances, such as pollen, pet dander and dust mites. Skin testing may help diagnose food allergies. But because food allergies can be complex, you may need additional tests or procedures.

Types of allergens

Allergens are substances that can cause an allergic reaction. There are three primary types of allergens:

  • Inhaled allergens affect the body when they come in contact with the lungs or membranes of the nostrils or throat. Pollen is the most common inhaled allergen.
  • Ingested allergens are present in certain foods, such as peanuts, soy, and seafood.
  • Contact allergens must come in contact with your skin to produce a reaction. An example of a reaction from a contact allergen is the rash and itching caused by poison ivy.

Allergy tests involve exposing you to a very small amount of a particular allergen and recording the reaction.

Insect sting allergy tests 

Why allergy testing is performed

Allergies affect more than 50 million people living in the USA, according to the American College of Allergy, Asthma, and Immunology. Inhaled allergens are by far the most common type. Seasonal allergies and hay fever, which is an allergic response to pollen, affect more than 40 million Americans.

The World Allergy Organization estimates that asthma is responsible for 250,000 deaths annually. These deaths can be avoided with proper allergy care, as asthma is considered an allergic disease process.

How allergy testing is performed

An allergy test may involve either a skin test or a blood test. You may have to go on an elimination diet if your doctor thinks you might have a food allergy.

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Skin tests

Skin tests are used to identify numerous potential allergens. This includes airborne, food-related, and contact allergens. The three types of skin tests are scratch, intradermal, and patch tests.

Your doctor will typically try a scratch test first. During this test, an allergen is placed in liquid, then that liquid is placed on a section of your skin with a special tool that lightly punctures the allergen into the skin’s surface. You’ll be closely monitored to see how your skin reacts to the foreign substance. If there’s localized redness, swelling, elevation, or itchiness of the skin over the test site, you’re allergic to that specific allergen.

If the scratch test is inconclusive, your doctor may order an intradermal skin test. This test requires injecting a tiny amount of allergen into the dermis layer of your skin. Again, your doctor will monitor your reaction.

Another form of skin test is the patch test (T.R.U.E. TESTTrusted Source). This involves using adhesive patches loaded with suspected allergens and placing these patches on your skin. The patches will remain on your body after you leave your doctor’s office. The patches are then reviewed at 48 hours after application and again at 72 to 96 hours after application.

Blood tests

If there’s a chance you’ll have a severe allergic reaction to a skin test, your doctor may call for a blood test. The blood is tested in a laboratory for the presence of antibodies that fight specific allergens. This test, called ImmunoCAP, is very successful in detecting IgE antibodies to major allergens.

Elimination diet

An elimination diet may help your doctor determine which foods are causing you to have an allergic reaction. It entails removing certain foods from your diet and later adding them back in. Your reactions will help determine which foods cause problems.

 

 

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