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Glypican-3 (GPC3), 1G12

Immunogen: Recombinant fragment containing amino acids 511-580 of human Glypican 3 (1G12); Full-length recombinant human GPC3 protein (GPC3 / 863)

Location: Cytoplasmic

Marker: Hepatocellular carcinoma marker

Specificity

Glypican-3 (GPC3) is an integral membrane protein that is mutated in Simpson-Golabi-Behmel syndrome (GBSS). SGBS is characterized by pre-and postnatal overgrowth and is an X-linked recessive condition. GPC3 can also be found in secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilms tumor.

In HCC patients, GPC3 is overexpressed in neoplastic liver tissue and elevated in serum, but is undetectable in normal liver, benign liver, and serum from healthy donors. GPC3 expression is also found to be higher in HCC liver tissue than in cirrhotic liver or liver with focal lesions such as dysplastic nodules and areas of liver adenoma (HA) with malignant transformation. In the context of testicular germ cell tumors, GPC3 expression is up-regulated in certain histological subtypes, specifically yolk sac tumors and choriocarcinoma.

 

A high level of GPC3 expression has also been found in some types of embryonal tumors, such as Wilm’s tumor and hepatoblastoma, with low or undetectable expression in adjacent normal tissue. In thyroid cancer patients, GPC3 expression is dramatically improved in certain types of cancers: 100% in follicular carcinoma and 70% in papillary carcinoma. The expression of GPC3 in follicular carcinoma was significantly higher than that of follicular adenoma. In contrast, GPC 3 is not expressed in anaplastic carcinoma.

Isotype: IgG

Clonality: Monoclonal

Host: Mouse

Gene: GPC3

Purity: Purified Protein A or G

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Applications / Dilutions

Dilutions

  • Flow cytometry 0.5 – 1 ug / million cells in 0.1 ml
  • Immunocytochemistry / Immunofluorescence 1-2 ug / ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 – 1.0 ug / ml
  • Immunofluorescence 0.5 – 1.0 ug / ml

Application notes

  • Immunohistochemistry (formalin-fixed): 1-2 ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1 mM EDTA buffer, pH 7.5-8.5, for 45 min at 95 ° C followed by cooling at RT for 20 min.
  • The optimal dilution for a specific application must be determined.

Theoretical MW

  • 67 kDa.
  • Disclaimer Note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post-translational modifications, post-translation cleavages, relative charges, and other experimental factors.

Packaging, storage, and formulations

Storage: Store at 4C.

Buffer: 10 mM PBS with 0.05% BSA

Preservative: 0.05% sodium azide

Concentration: 0.2mg / ml

Purity: Purified Protein A or G

Alternative names for the Glypican 3 antibody (1G12 + GPC3 / 863)

  • DGSX
  • Glypican 3
  • glypican 3 proteoglycan
  • glypican-3
  • GPC3
  • GTR2-2
  • proteoglycan heparan sulfate
  • OCI-5 intestinal protein
  • MXR7
  • OCI5
  • OCI-5
  • glypican-3 secreted
  • SGB
  • SGBS
  • SGBS1SDYS

Limitations

This product is for research use only and is not approved for human use or clinical diagnosis. Primary Antibodies are guaranteed for 1 year from the date of receipt.

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