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The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxypeptidases and has considerable homology to the human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7.

The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronavirus HCoV-NL63 and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2 (COVID-19 virus)

Nucleoprotein the positive strand of viral genome RNA into a helical ribbons leocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. It plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Coronavirus nucleoproteins are phosphoproteins and are encoded near the 3′ end of the genome.

N possesses two RNA-binding domains: an N-terminal domain with adjacent S/R-rich motif and the C-terminal 209 amino acids. N protein is involved in coronavirus infection in many ways: the C-terminal domain (CTD) of N is important for binding the genomic RNA packaging signal leading to selective genome incorporation, the N3 domain interacts with the endodomain of M to form virions, and the serine–arginine repeat region of N (SR) interacts with the first ubiquitin-like domain of nsp3 in a critical early replication step.

Moreover, it has also been demonstrated that N can oligomerize through interactions in the CTD, bind viral RNA through the N-terminal domain, unwind double-stranded nucleic acid in the manner of an RNA chaperone, and pack in a helix through the N-terminal domain, though none of these other functions has yet been demonstrated to be important for infection.

Product Details

• Description: Recombinant anti-SARS-CoV-2 Nucleoprotein Mouse ScFv is expressed from 293 cells (HEK293) with a human IgG1 Fc tag on C-terminal.
• Uniprot No: P0DTC9
• Alternative Names: Nucleocapsid protein, NC, protein N, N
• Species Reactivity: Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
• Immunogen Recombinant: Human Novel Coronavirus Nucleoprotein (N) (1-419aa) (CSB-EP3325GMY)
• Immunogen: Species Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
• Conjugate: Non-conjugated
• Clonality: Monoclonal
• Isotype Mouse scFv fusion with human IgG1 Fc
• Clone No: 1A6
• Purification Method: Affinity-chromatography
• Concentration It differs from different batches. Please contact us to confirm it.
• Buffer Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Form: Liquid
• Tested Applications: ELISA, WB, GICA

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time: Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing ways or locations, please kindly consult your local distributors for specific delivery time.

CSB-EAP33255A4 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). An antibody specific for SARS-CoV-2 Nucleoprotein (N) has been pre-coated onto the microwells. The SARS-CoV-2 Nucleoprotein (N) protein in samples is captured by the coated antibody after incubation.

Following extensive washing, another antibody Biotin conjugated specific for SARS-CoV-2 Nucleoprotein (N) is added to detect the captured SARS-CoV-2 Nucleoprotein (N) protein.

Followed by Tetramethyl-benzidine (TMB) reagent. The solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Product Details

• Alternative Names Nucleocapsid protein, NC, protein N, N
• Species Reactivity Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
• Immunogen Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa)
• Immunogen Species Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
• Concentration It differs from different batches. Please contact us to confirm it.
• Form Liquid
• Protein Names Human Novel Coronavirus Nucleoprotein (N)
• Host Capture: Mouse
• Detection: Mouse
• Buffer Capture: 50% Glycerol, 0.01M PBS, PH 7.4
• Detection: 50% Glycerol, 0.01M PBS, PH 7.4
• Components Capture: CSB-EAP33255A4C
• Detection: CSB-EAP33255A4D(Biotin) Reagents are sufficient for at least 5 x 96 well plates using recommended protocol.
• Tested Applications S-ELISA

Notes: We recommend using the capture antibody at a concentration of 1ug/ml and the detection antibody at a concentration of 0.05ug/ml. Optimal dilutions should be determined experimentally by the researcher.

Lead Time: Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing ways or locations, please kindly consult your local distributors for specific delivery times.