lyo NZYSupreme qPCR Probe Master Mix
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lyo NZYSupreme qPCR Probe Master Mix

Description: Lyo NZYSupreme qPCR Probe Master Mix (2x) is an optimized and highly efficient freeze-dried reaction mixture developed for realtime PCR. This master mix was engineered with a dual hot-start enzyme control mechanism to provide the highest detection sensitivity. In addition, the latest developments in PCR enhancers have been incorporated in the Lyo NZYSupreme qPCR Probe Master Mix, including buffer chemistry and incorporation of highly robust engineered enzymes. This master mix does not contain ROX and it was specifically developed for probe-detection technology, including molecular beacons. For qPCR instruments that require ROX reference dye, please add ROX (Cat. No. MB406) according to the table presented in the section “ROX reference dye”. Lyo NZYSupreme qPCR Probe Master Mix (2x) is provided as a simple-to-use, stabilized 2x reaction mixture that includes all components for quantitative PCR, except sample DNA, primers, probe and water.

Features:
– Eco-friendly room temperature shipment
– Stable at room temperature for 1 month
– Dual hot-start mode
– Ultra-sensitive: detects low-copy number targets
– Batch-to-batch reproducibility
– Intra-batch reproducibility
– Simple and reproducible
– Compatible with multiple real-time platforms

Components:
– Lyo NZYSupreme qPCR Probe Master Mix (2x)
– qPCR master mix reconstitution buffer

Applications:
– Real-time qPCR
– Two-step RT-qPCR
– Developed for probe-detection technology

New Lyo qPCR Probe Master Mix (2x)

Lyo NZYSupreme qPCR Probe Master Mix (2x)

Description: Lyo NZYSupreme qPCR Probe Master Mix (2x) is an optimized and highly efficient freeze-dried reaction mixture developed for realtime PCR. This master mix was engineered with a dual hot-start enzyme control mechanism to provide the highest detection sensitivity. In addition, the latest developments in PCR enhancers have been incorporated in the Lyo NZYSupreme qPCR Probe Master Mix, including buffer chemistry and incorporation of highly robust engineered enzymes. This master mix does not contain ROX and it was specifically developed for probe-detection technology, including molecular beacons. For qPCR instruments that require ROX reference dye, please add ROX (Cat. No. MB406) according to the table presented in the section “ROX reference dye”. Lyo NZYSupreme qPCR Probe Master Mix (2x) is provided as a simple-to-use, stabilized 2x reaction mixture that includes all components for quantitative PCR, except sample DNA, primers, probe and water.

lyo NZYSupreme qPCR Probe Master Mix
lyo NZYSupreme qPCR Probe Master Mix

Features:
– Eco-friendly room temperature shipment
– Stable at room temperature for 1 month
– Dual hot-start mode
– Ultra-sensitive: detects low-copy number targets
– Batch-to-batch reproducibility
– Intra-batch reproducibility
– Simple and reproducible
– Compatible with multiple real-time platforms

Components:
– Lyo NZYSupreme qPCR Probe Master Mix (2x)
– qPCR master mix reconstitution buffer

AceQ qPCR Probe Master Mix

Q112-03 2500 rxn (20 μl/rxn)
EUR 646

HotTaq Probe qPCR Mix (ROX)

BT11001 250rxn
EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq Probe qPCR Mix (Capillary)

BT11003 250rxn
EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq Probe qPCR Universal Mix

BT11004 250rxn
EUR 123
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

AceQ U+ Probe Master Mix

Q113-02 500 rxn (20 μl/rxn)
EUR 267

AceQ U+ Probe Master Mix

Q113-03 2500 rxn (20 μl/rxn)
EUR 842

SYBR Green qPCR Master Mix

HY-K0501 5 mL (500 rxns)
EUR 263

RT Master Mix for qPCR

HY-K0510 1 mL (100 rxns)
EUR 291

HotTaq Probe qPCR Mix (no ROX)

BT11002 250rxn
EUR 109
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

Fast Probe Master Mix (200 rxn)

31005 2x1ML
EUR 234
Description: Minimum order quantity: 1 unit of 2x1ML

Fast Probe Master Mix (500 rxn)

31005-1 5x1ML
EUR 466
Description: Minimum order quantity: 1 unit of 5x1ML

Fast Probe Master Mix (5000 rxn)

31005-2 50x1ML
EUR 3871
Description: Minimum order quantity: 1 unit of 50x1ML

ChamQ Geno-SNP Probe Master Mix

Q811-02 500 rxn (20 μl/rxn)
EUR 277

ChamQ Geno-SNP Probe Master Mix

Q811-03 2500 rxn (20 μl/rxn)
EUR 923

2x SYBR Green qPCR Master Mix

B21202 5 mL
EUR 224
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency.

2x SYBR Green qPCR Master Mix

B21203 25 mL
EUR 856
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency.

AceQ Universal SYBR qPCR Master Mix

Q511-02 500 rxn (20 μl/rxn)
EUR 221

AceQ Universal SYBR qPCR Master Mix

Q511-03 2500 rxn (20 μl/rxn)
EUR 646

ChamQ Universal SYBR qPCR Master Mix

Q711-02 500 rxn (20 μl/rxn)
EUR 221

ChamQ Universal SYBR qPCR Master Mix

Q711-03 2500 rxn (20 μl/rxn)
EUR 646

Accuris qMax Probe No Rox qPCR Mix

PR2001-N-1000 1 PC
EUR 467.74
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

AceQ U+ Universal Probe Master Mix V2

Q513-02 500 rxn (20 μl/rxn)
EUR 267

AceQ U+ Universal Probe Master Mix V2

Q513-03 2500 rxn (20 μl/rxn)
EUR 842

SYBR Green qPCR Master Mix (High ROX)

HY-K0521 1 mL (100 rxns)
EUR 113

SYBR Green qPCR Master Mix (Low ROX)

HY-K0522 5 mL (500 rxns )
EUR 257

SYBR Green qPCR Master Mix (No ROX)

HY-K0523 5 mL (500 rxns )
EUR 257

miRNA Universal SYBR® qPCR Master Mix

MQ101-01 125 rxn(20 μl/rxn)
EUR 138

miRNA Universal SYBR® qPCR Master Mix

MQ101-02 500 rxn(20 μl/rxn)
EUR 242

 

 

t.spot covid
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t.spot covid

HIGHLIGHTS

  • TSPOT.COVID is an ELISpot interferon gamma-release assay for SARS-CoV-2
  • TSPOT.COVID identifies a T cell response to SARS-CoV-2 spike S1 and N peptides
  • 2–8 weeks post SARS-CoV-2 diagnosis TSPOT.COVID detected 98% of infections
  • In comparison, immunoglobulin G (IgG) serology detected 83% of infections in the same period
  • Cellular immune response activated sooner and lasted longer than antibodies

Abstract

Objective

To evaluate the performance of the T-SPOT.COVID test for identifying SARS-CoV-2-responsive T-cells in participants with SARS-CoV-2 infection.

Methods

The T-SPOT.COVID test uses ELISpot interferon-gamma release assay (IGRA) methodology to measure T cell responses to SARS-CoV-2 spike S1 and nucleocapsid peptides. T-SPOT.COVID and anti-N immunoglobulin (Ig) G serology tests were performed on blood from 186 patients with nucleic acid amplification test (NAAT)-confirmed-SARS-CoV-2 infection and 100 control group participants.

Results

In the 2–8 weeks after NAAT-diagnosed SARS-CoV-2 infection, the T-SPOT.COVID test detected 98.4% (63 of 64) of infected participants, while anti-N IgG serology detected 82.8%. In the first 2 weeks after diagnosis, during adaptive immune response activation, there were less reactive T-SPOT.COVID responses (75.7%, 28 of 37 infected participants) and many less seropositive responses (32.4%). Response numbers tapered after 8 weeks; however, T-SPOT.COVID test continued to detect most participants with confirmed infection (83.6%, 56 of 67) and continued to out-perform serology (52.2%). T-SPOT.COVID response due to cross-reactive T cells was ruled out by demonstrating that, of 44 control group participants with T cells responsive to 4 human common cold coronavirus peptides, only 1 was T-SPOT.COVID reactive.

Conclusion

The T-SPOT.COVID test performed well in detecting SARS-CoV-2-sensitized T-cells over many months

Introduction

Long-term protection from infectious agents, such as the SARS-CoV-2 virus, is mediated by T cells and antibody-mediated immunity of the adaptive immune system (

Sette and Crotty, 2021

). The T-SPOT.COVID test was developed to identify the presence of SARS-CoV-2-responsive T cells.

T cells contribute to the understanding of SARS-CoV-2 infections in many ways. T cells can identify past SARS-CoV-2 infections at a time when PCR tests would be negative and antibodies levels may be waning (

Dan et al., 2021

;

Gudbjartsson et al., 2020

;

Poland et al., 2020

). T cells can provide immune memory lasting for months (

Dan et al., 2021

) and perhaps years, as suggested by the discovery of T cells to the SARS-CoV-1 coronavirus 17 years after infection (

Le Bert et al., 2020

). T cells may act independently of antibodies to control a SARS-CoV-2 infection, as shown by the recovery of COVID-19 patients who lack detectable antibodies but have SARS-CoV-2-responsive T cells (

Gallais et al., 2021

;

Sekine et al., 2020

). T cells also show reactivity to numerous SARS-CoV-2 epitopes, so have the potential to protect against many SARS-CoV-2 variants (

Grifoni et al., 2020

;

Tarke et al., 2021

). T cell-based assays can probe the longevity of an immune response following a SARS-CoV-2 infection or vaccination (

Goletti et al., 2021

;

Liu et al., 2021

;

Reynolds et al., 2021

). These various roles suggest that a T cell assay can be a key contributor to SARS-CoV-2 investigations.

The T-SPOT.COVID test, an enzyme-linked immunospot (ELISpot) assay, identifies T cells in peripheral blood that release interferon-gamma (IFN-γ) in response to stimulation with SARS-CoV-2 peptides. The T-SPOT.COVID test builds on the T-SPOT platform (Oxford Immunotec) used worldwide for tuberculosis and cytomegalovirus testing and the research version, the T-SPOT Discovery SARS-CoV-2 test (

Liu et al., 2021

;

t.spot covid
t.spot covid
Wyllie et al., 2021

). The T-SPOT.COVID ELISpot methodology is performed in many laboratories and offers a standardized comparison of T cell immunity among participants. In addition, ELISpot assays normalize the number of peripheral blood mononuclear cells (PBMCs), thus maintaining test effectiveness in participants with lymphopenia, a commonly reported condition in many COVID-19 patients (

Altmann and Boyton, 2020

) and immunosuppressed people.

The objective of this study was to evaluate the ability of the T-SPOT.COVID test to detect T cell responses in participants with or without a history of SARS-CoV-2 infection and to compare the T-SPOT.COVID test results with anti-N immunoglobulin (Ig)G serology results in the first several months after infection.

Materials and Methods

2.1 Participant recruitment

Participants for this single-center, cross-sectional study were recruited from patients who had attended the outpatient Primacare medical center in Fall River, Massachusetts, USA, between November 30, 2020, and March 24, 2021, a time of high demand for COVID-19 testing. Among other healthcare services, Primacare provided COVID-19 testing to anyone wanting or required to be tested. The New England Center for Clinical Research (NECCR) invited participants to join the study if they had received a positive SARS-CoV-2 nucleic acid amplification test (NAAT) at Primacare or if NECCR deemed them to be at low risk of SARS-CoV-2 infection. As this study was run independently from the participants’ healthcare providers, clinical data such as chest x-rays and hospitalizations records were not obtained. Informed consent and study approval were obtained from the Advarra institutional review board by NECCR at Primacare.
Confirmed-infection group: A NAAT, which detects the presence of the SARS-CoV-2 virus, was used to identify people infected with SARS-CoV-2 at the time of testing (

Rai et al., 2021

). Participants in the confirmed-infection group were recruited from asymptomatic and symptomatic patients who had had a positive SARS-CoV-2 NAAT result within the past 9 months. The date of the first positive NAAT result was considered the date of diagnosis of SARS-CoV-2 infection. Blood was drawn for Abbott SARS-CoV-2 chemiluminescent microparticle immunoassay (CMIA) anti-N IgG serology and T-SPOT.COVID tests between 0 to 249 days after diagnosis.

The analysis of responses was divided into 3 time periods: 0 to 2 weeks after diagnosis (0 to 14 days); 2+ to 8 weeks after diagnosis (15 to 56 days); and 8+ weeks after diagnosis (57+ days).
Control group: Many SARS-CoV-2 studies use frozen pre-pandemic blood for control samples; however, the T-SPOT platform requires fresh blood to ensure consistent results. Therefore fresh blood was obtained from control group participants prospectively recruited from individuals with low risk of prior SARS-CoV-2 infection. Requirements for enrollment included no current or prior signs or symptoms of COVID-19, no known contact with a confirmed SARS-CoV-2-infected individual, no prior history of a positive SARS-CoV-2 NAAT, no SARS-CoV-2 vaccination, and no prior diagnosis with SARS-CoV-1 or Middle Eastern Respiratory Syndrome (MERS). In addition, the BIOHIT HealthCare SARS-CoV-2 lateral flow anti-N IgM/IgG serology test was performed at enrollment, and the 1 person with a positive BIOHIT result was not enrolled. Blood was drawn at enrollment for testing with T-SPOT.COVID and the Abbott CMIA anti-N IgG serology test and anyone with a positive serology result was excluded from the control group.

2.2 T-SPOT.COVID test

The T-SPOT.COVID test includes over 250 SARS-CoV-2 peptides (15-mer peptides overlapping by 11 amino acids) in 2 antigen peptide pools; one pool contains peptides from the spike S1 protein, including the receptor-binding domain, and the other contains peptides from the nucleocapsid protein.
Blood samples for the T-SPOT.COVID test were processed and analyzed according to the manufacturer’s instructions. Briefly, blood samples were drawn into lithium heparin tubes which were shipped overnight to Oxford Immunotec (Abingdon, UK) in temperature-controlled shipping boxes. Next, the T-Cell Xtend reagent (Oxford Immunotec) was added to the samples, and PBMCs were isolated by density gradient centrifugation, washed, counted, and 250 000 cells/well were plated into 4 wells of a 96-well plate.

COVID-19 IgG/IgM Rapid Test Kit

UNCOV-20 20T/kit
EUR 155

Accu-Tell COVID-19 IgG/IgM Rapid Test

GEN-B352-20tests 20 tests
EUR 236
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

Accu-Tell COVID-19 IgG/IgM Rapid Test

GEN-B352-40tests 40 tests
EUR 321
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

Panbio™ COVID-19 IgG/IgM Rapid Test

ICO-T402 25 Tests/Kit
EUR 220

Panbio™ COVID-19 Ag Rapid Test Device

41FK10 25 Tests/Kit
EUR 138

Purified recombinant COVID-19 (isolate Wuhan-Hu-1) NP protein

nCoVNP-126V 100ug
EUR 792
Description: Purified recombinant COVID-19(isolate Wuhan-Hu-1) NP protein was expressed in HEK293 cells.

Recombinant COVID-19 (isolate Wuhan-Hu-1) S(ΔTM) protein

nCoVS-125V 100ug
EUR 792
Description: Purified recombinant COVID-19(isolate Wuhan-Hu-1) S(ΔTM) protein was expressed in HEK293 cells.

Novel Coronavirus COVID-19 (2019-nCoV) Real Time RT-PCR Kit

RR-0478-02 25 tests/kit
EUR 991
  • For use with PE5700, MJ-Opticon & other single color systems, ABI7000, ABI7300, ABI7500, ABI7900, ABI StepOne, StepOne plus, MJ-Opticon2, MJ-chromo4, MX3000P, MX3005P, Smart Cycler II, Rotor-Gene 6000, LightCycler 480, CFX 96, Life 96, Slan 96, iCycl
  • Show more
Description: Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems.

Novel Coronavirus COVID-19 (2019-nCoV) Real Time Multiplex RT-PCR Kit (Detection for 3 Genes )

RR-0479-02 25 tests/kit
EUR 1347
  • For use with PE5700, MJ-Opticon & other single color systems, ABI7000, ABI7300, ABI7500, ABI7900, ABI StepOne, StepOne plus, MJ-Opticon2, MJ-chromo4, MX3000P, MX3005P, Smart Cycler II, Rotor-Gene 6000, LightCycler 480, CFX 96, Life 96, Slan 96, iCycl
  • Show more
Description: Novel Coronavirus (2019-nCoV) Real Time Multiplex RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems. It detects N gene, E gene and RdRp gene of 2019-nCoV. RR-0479-02 has been also approverd by CFDA for emergency use and is WHO standard.