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Mouse IgG (H and L chains) Peroxidase Monoclonal Antibody

Monoclonal antibodies are created by infusing an antigen into a host creature to start a humoral safe reaction. In many methodology, spleen cells from these hosts are combined in vitro with refined harmful myeloma cells. Remarkable cell clones are secluded and those that endure the combination step are known as hybridomas. Hybridomas are godlike as a result of their myeloma attributes and are effortlessly proliferated in culture. Due to their B cell properties, some hybridoma clones proceed to combine and emit hereditarily homogeneous antibodies against a solitary epitope, called monoclonal antibodies. Monoclonal antibodies are homologous to regular immunoglobulins from the source vaccinated creature, yet dissimilar to polyclonal antibodies cleaned from serum, they are well defined for a solitary epitope and give a stable long haul supply of delivered by hybridomas in vitro. We offer a wide scope of monoclonal antibodies for use in different applications.

Sera from mouse

Sera from mouse has been utilized to obstruct vague antigen restricting in immunohistochemistry. It has additionally been utilized as a control in AdV killing counter acting agent measure.
Mouse serum is utilized in an assortment of mouse cell culture frameworks to concentrate on viral disease, hindrance and transduction processes.

What is the class (isotype) and additionally subclass of the essential counter acting agent?

This question is basically significant while working with monoclonal antibodies. Polyclonal antibodies, be that as it may, are normally IgG class immunoglobulins. Hence, the optional antibodies will chiefly be an enemy of IgG counter acting agent.

Monoclonal antibodies are most usually evolved in mice and every so often in rodents, hamsters, or bunnies. For instance, assuming the essential monoclonal neutralizer is mouse IgM, one would need an optional immune response that responds with mouse IgM (hostile to Mouse IgM).

In the event that the essential monoclonal is one of the mouse IgG subclasses, practically any enemy of mouse IgG optional immune response ought to tie to it. In the event that the subclass of the essential immunizer isn’t known, then enemy of Mouse IgG F(ab) auxiliary antibodies might be utilized since they perceive most mouse immunoglobulin subclasses.

There are many classes and subclasses of human and mouse IgG(s). Picking an optional might be troublesome. Be that as it may, one normal element among these IgG(s) are the light chains (kappa and lambda). At the end of the day, IgG, IgM, IgA, IgD and IgE all have either kappa or lambda light chains. The weighty chain, nonetheless, is class explicit.

In what species was the essential immunizer created?

Optional antibodies are coordinated against the types of the essential neutralizer. Accordingly, you will require an auxiliary counter acting agent that is brought up in an animal varieties not the same as the host types of the essential neutralizer. For instance, assuming that your essential immune response is brought up in a mouse, you will require an enemy of mouse auxiliary immunizer brought up in goat, bunny, and so on.

Optional Antibodies

Optional antibodies are polyclonal or monoclonal antibodies that tight spot to essential antibodies or neutralizer parts, like the Fc or Fab locales. They are regularly named with tests that make them helpful for discovery, decontamination, or arranging applications.
We offer optional antibodies from an assortment of host animal varieties. Our polyclonal optional antibodies are delivered from the serum of host creatures like mice, hares, goats, and sheep. While, our monoclonal optional antibodies are delivered from mouse hybridoma clones.

Formed ANTIBODIES AND PROBES

We offer a huge arrangement of formed antibodies. A formed counter acting agent is a monoclonal or polyclonal immunizer connected to a mark and utilized for recognition in an assorted scope of measure procedures. The particular utility of an optional immunizer relies on its formed probe(s). Tests are atoms that help different identification innovations. The most widely recognized discovery frameworks for formed optional antibodies are colorimetric or fluorescent.

Colorimetric measures are commonly founded on the utilization of soluble phosphatase (ALP) or horseradish peroxidase (HRP) or its subordinates. The biotin avidin (streptavidin) form restricting framework is frequently used to enhance the colorimetric sign for ALP or HRP. The most well-known fluorescent measures use fluorescein isothiocyanate (FITC), Rhodamine or its subsidiary, tetramethylrhodamine isothiocyanate (TRITC), cyanine (Cy3), or phycoerythrin (R-PE).

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Control Genomic DNA - Bovine Female
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Dog Genomic DNA, Female
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Rabbit Genomic DNA, Female
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Chicken Genomic DNA, Female
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Porcine Genomic DNA, Female
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Rat Wistar Genomic DNA, Female
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Rat Fischer Genomic DNA, Female
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Frog Xenopus Genomic DNA. Female
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Hamster, Chinese, Genomic DNA, Female*
GA-170CF Zyagen 0.05mg 177 EUR

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GFS-190F Zyagen 0.05mg 177 EUR

Hamster, Armenian, Genomic DNA, Female
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Control Genomic DNA - Rat Female
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Control Genomic DNA - Dog Female
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Control Genomic DNA - Horse Female
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We offer formed antibodies connected to an assortment of colorimetric and fluorescent names for use in recognition, filtration, arranging, and microscopy applications. Formed antibodies are delivered in an assortment of host creatures making them viable with a wide scope of immunochemical reagents. For those clients whose examination requires marking their own reagents, we offer neutralizer naming units for the formation of different names (biotin, FITC, CF™ names) to monoclonal and polyclonal antibodies.

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Mouse IgG (H and L chains) Peroxidase Monoclonal Antibody.

TMB  is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a dark blue tone during the enzymatic debasement of hydrogen peroxide by HRP, and the expansion of a stop arrangement gives an unmistakable yellow variety that assimilates at 450 nm. This substrate gives higher sign and has a higher responsiveness in ELISA than OPD (ortho-phenylenediamine dichloride).

TMB PLUS2 attributes are:

  • Medium awareness
  • Exceptionally low foundation
  • Watery based – no natural solvents (REACH Annex XVII agreeable)
  • Low parcel to-part variety
  • For recognizable pipetting, we suggest utilizing pink shaded TMB PLUS2 Prestained (feline. no. 4445). The pink marker vanishes after expansion of the stop arrangement.

Biologics

Organic items, like helpful proteins, monoclonal antibodies, immunizations, and cell and quality treatment items have become progressively significant. Not at all like customary artificially incorporated drugs that follow up on a hydrophobic pocket of a protein to initiate or restrain a kinase pathway, biotherapeutics pharmacological activity is by means of restricting to its objective or immunomodulation.

Because of the mind boggling nature of organic items, researchers experience various difficulties from early medication revelation through improvement. For instance, during recognizable proof of a clone with the ideal immunogenicity that can show restricting explicitness to the objective and resulting adjustment or get the ideal safe reaction, and further during clone portrayal, increase, and creation of these items.

From revelation through assembling, tackling enormous atom medications to make protected and successful treatments is perplexing and costly. PerkinElmer empowers researchers to create and smooth out their whole biologics work process so they can defeat these difficulties and bring reliable, superior grade, organic medications to showcase quicker.

Biomolecular Discovery.

Biotherapeutics drug revelation, similar as customary little atom drug disclosure, begins with approval of the helpful objective. Much of the time approving the objective means there is an adequate number of information to relate the objective to an infection state, and the objective can be regulated with a biologic helpful.

The beginning phases of medication revelation are basic and require information driven forward leaps, computerized work processes to improve on complex examination to save time and permit researchers to concentrate in the new organic substance (NBE) disclosure and early portrayal to empower accomplishment during drug advancement.

Our specialists can plan your computerization needs from nucleic corrosive segregation to quality articulation examination accoupled with cutting edge measure stages considering symmetrical hit affirmation involving various sciences in a high throughput design, speeding up your way from target ID to lead disclosure.

In what species was the essential immunizer created?

Optional antibodies are coordinated against the types of the essential counter acting agent. In this manner, you will require an optional neutralizer that is brought up in an animal varieties not quite the same as the host types of the essential counter acting agent. For instance, on the off chance that your essential immunizer is brought up in a mouse, you will require an enemy of mouse auxiliary neutralizer brought up in goat, hare, and so on.

What is the class (isotype) or potentially subclass of the essential immune response?

This question is essentially significant while working with monoclonal antibodies. Polyclonal antibodies, notwithstanding, are regularly IgG class immunoglobulins. Consequently, the auxiliary antibodies will fundamentally be an enemy of IgG immune response.

Monoclonal antibodies are most ordinarily evolved in mice and every so often in rodents, hamsters, or bunnies. For instance, assuming the essential monoclonal immunizer is mouse IgM, one would need an optional counter acting agent that responds with mouse IgM (hostile to Mouse IgM).

In the event that the essential monoclonal is one of the mouse IgG subclasses, practically any enemy of mouse IgG auxiliary immunizer ought to tie to it. On the off chance that the subclass of the essential immunizer isn’t known, then, at that point, hostile to Mouse IgG F(ab) optional antibodies might be utilized since they perceive most mouse immunoglobulin subclasses.

Dog Universal Reference cDNA-Oligo dT
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Monkey Universal Reference cDNA-Oligo dT
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Dog Universal Reference cDNA-Random Hexamer
DD-UR-40-RH Zyagen 1x40 reactions 280 EUR

There are many classes and subclasses of human and mouse IgG(s). Picking an optional might be troublesome. In any case, one normal element among these IgG(s) are the light chains (kappa and lambda). At the end of the day, IgG, IgM, IgA, IgD and IgE all have either kappa or lambda light chains. The weighty chain, in any case, is class explicit.

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Cell Counting Kit-8 (CCK-8)

Cell Counting Kit-8 (CCK-8) permits helpful tests utilizing WST-8 (2-(2-methoxy-4-nitrophenyl)- 3-(4-nitrophenyl)- 5-(2,4-disulfophenyl)- 2H-tetrazolium, monosodium salt), which delivers a water-dissolvable formazan color upon bioreduction within the sight of an electron transporter, 1-Methoxy PMS. CCK-8 arrangement is added straightforwardly to the cells, no pre-blending of parts is required. WST-8 is bioreduced by cell dehydrogenases to an orange formazan item that is solvent in tissue culture medium.

How much formazan created is straightforwardly corresponding to the quantity of living cells. Since the CCK-8 arrangement is entirely steady and it has little cytotoxicity, a more extended brooding, like 24 to 48 hours, is conceivable.
Cell Counting Kit-8 permits touchy colorimetric examines for the assurance of the quantity of reasonable cells in the multiplication and cytotoxicity tests. The recognition responsiveness is higher than some other tetrazolium salts like MTT, XTT or MTS.

Cell Counting Kit 8, ccK-8 for short, is a profoundly delicate and non-radioactive colorimetric identification strategy used to decide the quantity of living cells in Cell multiplication or harmfulness tests.

Cck-8 is an update result of MTT. Cck-8 arrangement can be straightforwardly added to cell tests without prepreparation of different parts, fast recognition and extremely low poisonousness. Cck-8 depends on the water-dissolvable tetrazole salt WST-8.

This is the secret: WST-8, within the sight of an electron-coupled reagent, can be decreased by a chemical called dehydrogenase in the mitochondria to create an orange messy color. Nail dark breaks down in tissue media with respect to the quantity of living cells. By colorimetry, the quantity of living cells can be progressively evaluated to recognize cell multiplication or medication poisonousness. This unit is ordinarily used to identify cycle and apoptosis of connected or suspended cells. On account of tissue, the tissue should be processed into a solitary cell state before it tends to be tried.

The TC20 mechanized cell counter includes mammalian cells in a single basic advance utilizing its inventive auto-center innovation and complex cell counting calculation to create exact cell includes in under 30 seconds. Endless supply of an including slide, the TC20 cell counter quickly gives an absolute cell count (no matter what trypan blue staining) and surveys cell practicality by means of trypan blue rejection..

Key Features and Benefits

  • Viable with an expansive scope of cell sizes and types – counts cell lines, essential cells (from tissue or blood), and undifferentiated organisms
  • Creative auto-center innovation – eliminates the variety related with manual centering and prompts exact cell includes in 30 seconds
  • Cell size entryways – client chooses a populace of interest in complex examples, like essential cells, or lets the cell counting calculation accomplish practically everything
  • Cell practicality – investigates cells precisely utilizing multifocal plane examination
  • Simple to file and investigate – accumulates to 100 includes in the installed memory for access any time, or utilize the discretionary TC20 information analyzer programming on your PC to additionally break down sent out cell pictures
  • Getting every one of the information you really want about your cell societies is quick and simple; the TC20 cell counter and expendable counting slides dispense with the requirement for dreary arrangement, cleaning, or upkeep.
  • The TC20 cell counter is straightforward and instinctive to utilize – figure out how to utilize the TC20 mechanized cell counter in the presentation video or step through a virtual examination drive.

Come by Accurate and Reproducible Results

The TC20 robotized cell counter purposes microscopy with auto-center that examines various central planes to distinguish the best plane. Without requiring any client input, the refined cell counting calculation utilizes the picture obtained from the best central plane to distinguish cells and prohibit trash, consequently working out the complete cell count.

Cell Counting Kit-8
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Cell Counting Kit-8
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Cell Counting Kit-8
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Cell Counting Kit-8 (CCK-8)
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Cell Counting Kit-8 (CCK-8)
KTA1020-10000T Abbkine 10000 T 549 EUR

The auto-center prompts profoundly reproducible cell counts with decreased client to-client inconstancy contrasted with a hemocytometer and cell counters with manual concentration. Utilizing auto-center rather than emotional manual centering is particularly significant while surveying cell reasonability on the grounds that an erroneously chosen central plane will prompt wrong outcomes.

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Mouse Anti-Zika Virus NS1 Antibody (D11):Biotin Conjugate.

Genome association of SARS-CoV-2 is like that of SARS-CoV, with both being coordinated into two principle open understanding edges (ORFs) and a few more modest downstream ORFs. Two enormous ORFs, ORF1a and ORF1b, encode two polyproteins which are separated by viral encoded proteases bringing about a few non-primary proteins (nsp). ORF1a encodes a 440-500 kDa polypeptide (pp1a) which is enzymatically handled to produce 11 nsps. The second ORF, ORF1b, encodes a bigger polypeptide (pp1ab) of 740-810 kDa which is separated to create 16 nsps.

What are SARS-CoV-2 Structural Proteins?

Four significant primary proteins have been recognized in SARS-CoV-2 including spike, nucleocapsid, layer, and envelope proteins, what share critical character with SARS-CoV. These four underlying proteins are encoded by ORF2-10 and are expected for viral coat arrangement and genome encapsidation. Contrasted with nsps, underlying proteins get higher insusceptible reactions, both humoral and cell interceded.

SARS-CoV-2 Spike Protein.

The spike protein in SARS-CoV-2 (1,273 aa) is a viral surface glycoprotein with two significant utilitarian spaces, the S1 (14-667 aa) and S2 (668-1255 aa) areas, which intervene cell receptor restricting and film combination, individually. Viral connection and section into have cells is subject to the communication of the spike’s receptor-restricting area with explicit cell proteins.

The angiotensin changing over protein 2 (ACE-2) and the protease TMPRSS2 have been recognized as the cell receptor and preparing protease expected for SARS-CoV-2 cell passage, separately. The protease TMPRSS2 or potentially cathepsin L separate the spike protein at the S1-S2 intersection, permitting the combination of viral envelope and cell films expected for viral passage. SARS-CoV-2 may likewise be divided by furin which perceives a four amino corrosive arrangement at the S1-S2 intersection. Other than cleavage of the spike protein at the S1-S2 intersection, cell proteases sever the S2 subunit at a perceived S2′ arrangement which is basic for actuation of the spike protein and ensuing layer combination.

How Spike Protein Sequences Compare Between SARS-CoV-2 versus SARS-CoV ?

The surface glycoprotein or spike protein of SARS-CoV-2 offers 76% arrangement character with SARS-CoV’s spike protein . Novus Biologicals offers a few antibodies for the identification of SARS-CoV spike protein which are approved in a few applications (e.g., ELISA, Flow, ICC/IF, IHC, IHC-P, IP, Simple Western, SPR, WB). Novus Innovator’s Reward Program permits you to test a portion of these accessible antibodies for the discovery of SARS-CoV-2. Also, through Novus’ 100 percent promise you can test antibodies against SARS-CoV-2 targets offering 90% or more prominent homology to proteins in SARS-CoV. Become familiar with our 100 percent assurance and Innovator’s Reward Program.

SARS-CoV-2 Nucleocapsid Protein.

The nucleocapsid phosphoprotein (419 aa) is situated inside the center of the SARS-CoV-2 viral molecule and connects with the viral RNA. During viral gathering, the nucleocapsid protein assumes a focal part in pressing the viral RNA genome. This cycle is reliant upon its capacity to self-partner not set in stone for the SARS-CoV nucleocapsid protein. SARS-CoV nucleocapsid protein has been embroiled in different capacities, for example, the balance of host cell processes including cell cycle liberation, hindrance of IFN creation, and acceptance of proinflammatory variables (e.g., COX-2).

How Nucleocapsid Protein Sequences Compare Between SARS-CoV-2 versus SARS-CoV?

SARS-CoV-2 nucleocapsid protein imparts 91% arrangement personality to the SARS-CoV protein . Novus Biologicals offers a few antibodies for the identification of SARS-CoV nucleocapsid protein which are approved in a few applications (e.g., ELISA, Flow, ICC/IF, IHC, IHC-P, IP, Simple Western, WB).

Basic Western investigation of (Left) recombinant SARS-CoV-2 Nucleocapsid Protein (10474-CV) with SARS Nucleocapsid Protein Antibody [NB100-56683]. SARS Nucleocapsid protein was stacked at 20 ng/mL and identified utilizing sequential weakenings of the Rabbit Anti-SARS-CoV Nucleocapsid Protein Polyclonal Antibody (NB100-56683) trailed by HRP-formed Anti-Goat IgG Secondary Antibody.

Simple Western investigation of SARS-CoV-2 (1:50), MERS (1:100), OC43 (1:100), and 229E (1:100) lysates. A particular band was identified for SARS-CoV-2 Nucleocapsid Protein just in the SARS-CoV-2 lysate. Location depended on the utilization of Rabbit Anti-SARS-CoV Nucleocapsid Protein Polyclonal Antibody [NB100-56683] followed by HRP-formed Anti-Goat IgG Secondary Antibody.

Note: some reactivity saw with FL Std 230. SARS-CoV-2 lysate politeness of University of Maryland. These trials were led under diminishing circumstances and utilizing the 12-230 kDa division framework.

Distribution Highlight: Development of a Rhesus Macaque Model of COVID-19.

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A new report fostered a creature model of SARS-CoV-2 contamination in rhesus macaques (presently additionally distributed by Nature “Respiratory sickness in rhesus macaques vaccinated with SARS-CoV-2 “) and used the bunny polyclonal hostile to SARS-CoV nucleocapsid counter acting agent [NB100-56576] to recognize the presence of the SARS-CoV-2 infection in tainted tissues effectively.

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lyo NZYSupreme qPCR Probe Master Mix

Description: Lyo NZYSupreme qPCR Probe Master Mix (2x) is an optimized and highly efficient freeze-dried reaction mixture developed for realtime PCR. This master mix was engineered with a dual hot-start enzyme control mechanism to provide the highest detection sensitivity. In addition, the latest developments in PCR enhancers have been incorporated in the Lyo NZYSupreme qPCR Probe Master Mix, including buffer chemistry and incorporation of highly robust engineered enzymes. This master mix does not contain ROX and it was specifically developed for probe-detection technology, including molecular beacons. For qPCR instruments that require ROX reference dye, please add ROX (Cat. No. MB406) according to the table presented in the section “ROX reference dye”. Lyo NZYSupreme qPCR Probe Master Mix (2x) is provided as a simple-to-use, stabilized 2x reaction mixture that includes all components for quantitative PCR, except sample DNA, primers, probe and water.

Features:
– Eco-friendly room temperature shipment
– Stable at room temperature for 1 month
– Dual hot-start mode
– Ultra-sensitive: detects low-copy number targets
– Batch-to-batch reproducibility
– Intra-batch reproducibility
– Simple and reproducible
– Compatible with multiple real-time platforms

Components:
– Lyo NZYSupreme qPCR Probe Master Mix (2x)
– qPCR master mix reconstitution buffer

Applications:
– Real-time qPCR
– Two-step RT-qPCR
– Developed for probe-detection technology

New Lyo qPCR Probe Master Mix (2x)

Lyo NZYSupreme qPCR Probe Master Mix (2x)

Description: Lyo NZYSupreme qPCR Probe Master Mix (2x) is an optimized and highly efficient freeze-dried reaction mixture developed for realtime PCR. This master mix was engineered with a dual hot-start enzyme control mechanism to provide the highest detection sensitivity. In addition, the latest developments in PCR enhancers have been incorporated in the Lyo NZYSupreme qPCR Probe Master Mix, including buffer chemistry and incorporation of highly robust engineered enzymes. This master mix does not contain ROX and it was specifically developed for probe-detection technology, including molecular beacons. For qPCR instruments that require ROX reference dye, please add ROX (Cat. No. MB406) according to the table presented in the section “ROX reference dye”. Lyo NZYSupreme qPCR Probe Master Mix (2x) is provided as a simple-to-use, stabilized 2x reaction mixture that includes all components for quantitative PCR, except sample DNA, primers, probe and water.

lyo NZYSupreme qPCR Probe Master Mix
lyo NZYSupreme qPCR Probe Master Mix

Features:
– Eco-friendly room temperature shipment
– Stable at room temperature for 1 month
– Dual hot-start mode
– Ultra-sensitive: detects low-copy number targets
– Batch-to-batch reproducibility
– Intra-batch reproducibility
– Simple and reproducible
– Compatible with multiple real-time platforms

Components:
– Lyo NZYSupreme qPCR Probe Master Mix (2x)
– qPCR master mix reconstitution buffer

Apex qPCR Probe Master Mix
42-118P 400 x 25µl Reactions/Unit
EUR 341.44
Description: 2X, Low ROX, 5ml

Apex qPCR Probe Master Mix
42-119P 4000 x 25µl Reactions/Unit
EUR 2926.74
Description: 2X, Low ROX, 50ml

Apex qPCR Probe Master Mix
42-120P 400 x 25µl Reactions/Unit
EUR 341.44
Description: 2X, High ROX, 5ml

Apex qPCR Probe Master Mix
42-121P 4000 x 25µl Reactions/Unit
EUR 2926.74
Description: 2X, High ROX, 50ml

AceQ qPCR Probe Master Mix
Q112-02 500 rxn (20 μl/rxn)
EUR 120.55

AceQ qPCR Probe Master Mix
Q112-03 2500 rxn (20 μl/rxn)
EUR 543.5

Probe qPCR Master Mix (2x)
E0420-01 100reactions
EUR 55.59
Description: For quantitative real-time PCR and two-step real-time RT-PCR Using sequence-specific probes

Probe qPCR Master Mix (2x)
E0420-02 200reactions
EUR 106.82
Description: For quantitative real-time PCR and two-step real-time RT-PCR Using sequence-specific probes

Probe qPCR Master Mix (2x)
E0420-03 1000reactions
EUR 467.61
Description: For quantitative real-time PCR and two-step real-time RT-PCR Using sequence-specific probes

Entrans 2X qPCR Probe Master Mix
RK21208 40 RXN
EUR 185.3

MethyLight 2X qPCR Probe Master Mix
RK21240 1mL
EUR 209.83

Entrans 2X qPCR Probe Master Mix V2
RK21212 50RXN
EUR 40.88

Fast Probe qPCR Master Mix (2x)
E0422-01 100reactions
EUR 55.59
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR Using sequence-specific probes

Fast Probe qPCR Master Mix (2x)
E0422-02 200reactions
EUR 106.82
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR Using sequence-specific probes

Fast Probe qPCR Master Mix (2x)
E0422-03 1000reactions
EUR 467.61
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR Using sequence-specific probes

BioEasy Multiplex qPCR Probe Master Mix II
TRI-B71M1 500T
EUR 619.92

BioEasy Multiplex qPCR Probe Master Mix II
TRI-B71S1 100T
EUR 138.6

Entrans 2X qPCR Probe Master Mix with UDG
RK21222 50RXN
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2X qPCR Probe Master Mix with Separate ROX
MBS308018-1000Reactions 1000Reactions
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2X qPCR Probe Master Mix with Separate ROX
MBS308018-100Reactions 100Reactions
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2X qPCR Probe Master Mix with Separate ROX
MBS308018-2500Reactions 2500Reactions
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2X qPCR Probe Master Mix with Separate ROX
MBS308018-500Reactions 500Reactions
EUR 405

HotStart™ 2X Probe qPCR Master Mix
K1541-25 25ml
EUR 437.6
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ 2X Probe qPCR Master Mix
K1541-5 5ml
EUR 123.2
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ 2X Probe qPCR Master Mix
K1541-50 50ml
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Description: Reverse Transcription & PCR|qPCR/Real-time PCR

igScript™ Probe-Based qPCR master mix
4233 500
EUR 172
Description: IgScript™ probe-based qPCR 2x master mix contains IgScript™ Taq DNA polymerase, MgCl2, dNTPs, stabilizers, enhancers and low ROX reference dye with standard buffer providing improved qPCR efficiency, wider dynamic range, superior sensitivity and specificity. IgScript™ qPCR 2x master mix is a ready-to-use cocktail containing all components except primers, probe and template, for the amplification and detection of DNA in qPCR. This 2x master mix requires minimal handling during reaction setup and offer consistent and robust qPCR reactions. Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase (1, 2) and a 5´→3´ exonuclease activity (3, 4). The amplification step features a high quality Taq DNA Polymerase which offers robust, reliable and better amplification.Product IncludesigScript™ Probe-Based qPCR master mixApplicationsGene expression data validation.MultiplexingMutation detectionPathogen and viral detectionGenetically modified organisms (GMO) characterization and Genetic profiling

igScript™ Probe-Based qPCR master mix
4235 1000
EUR 276
Description: IgScript™ probe-based qPCR 2x master mix contains IgScript™ Taq DNA polymerase, MgCl2, dNTPs, stabilizers, enhancers and low ROX reference dye with standard buffer providing improved qPCR efficiency, wider dynamic range, superior sensitivity and specificity. IgScript™ qPCR 2x master mix is a ready-to-use cocktail containing all components except primers, probe and template, for the amplification and detection of DNA in qPCR. This 2x master mix requires minimal handling during reaction setup and offer consistent and robust qPCR reactions. Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase (1, 2) and a 5´→3´ exonuclease activity (3, 4). The amplification step features a high quality Taq DNA Polymerase which offers robust, reliable and better amplification.Product IncludesigScript™ Probe-Based qPCR master mixApplicationsGene expression data validation.MultiplexingMutation detectionPathogen and viral detectionGenetically modified organisms (GMO) characterization and Genetic profiling

igScript™ Probe-Based qPCR master mix
4237 2500
EUR 559.2
Description: IgScript™ probe-based qPCR 2x master mix contains IgScript™ Taq DNA polymerase, MgCl2, dNTPs, stabilizers, enhancers and low ROX reference dye with standard buffer providing improved qPCR efficiency, wider dynamic range, superior sensitivity and specificity. IgScript™ qPCR 2x master mix is a ready-to-use cocktail containing all components except primers, probe and template, for the amplification and detection of DNA in qPCR. This 2x master mix requires minimal handling during reaction setup and offer consistent and robust qPCR reactions. Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase (1, 2) and a 5´→3´ exonuclease activity (3, 4). The amplification step features a high quality Taq DNA Polymerase which offers robust, reliable and better amplification.Product IncludesigScript™ Probe-Based qPCR master mixApplicationsGene expression data validation.MultiplexingMutation detectionPathogen and viral detectionGenetically modified organisms (GMO) characterization and Genetic profiling

 

 

Chase
t.spot covid
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t.spot covid

HIGHLIGHTS

  • •
    TSPOT.COVID is an ELISpot interferon gamma-release assay for SARS-CoV-2
  • •
    TSPOT.COVID identifies a T cell response to SARS-CoV-2 spike S1 and N peptides
  • •
    2–8 weeks post SARS-CoV-2 diagnosis TSPOT.COVID detected 98% of infections
  • •
    In comparison, immunoglobulin G (IgG) serology detected 83% of infections in the same period
  • •
    Cellular immune response activated sooner and lasted longer than antibodies

Abstract

Objective

To evaluate the performance of the T-SPOT.COVID test for identifying SARS-CoV-2-responsive T-cells in participants with SARS-CoV-2 infection.

Methods

The T-SPOT.COVID test uses ELISpot interferon-gamma release assay (IGRA) methodology to measure T cell responses to SARS-CoV-2 spike S1 and nucleocapsid peptides. T-SPOT.COVID and anti-N immunoglobulin (Ig) G serology tests were performed on blood from 186 patients with nucleic acid amplification test (NAAT)-confirmed-SARS-CoV-2 infection and 100 control group participants.

Results

In the 2–8 weeks after NAAT-diagnosed SARS-CoV-2 infection, the T-SPOT.COVID test detected 98.4% (63 of 64) of infected participants, while anti-N IgG serology detected 82.8%. In the first 2 weeks after diagnosis, during adaptive immune response activation, there were less reactive T-SPOT.COVID responses (75.7%, 28 of 37 infected participants) and many less seropositive responses (32.4%). Response numbers tapered after 8 weeks; however, T-SPOT.COVID test continued to detect most participants with confirmed infection (83.6%, 56 of 67) and continued to out-perform serology (52.2%). T-SPOT.COVID response due to cross-reactive T cells was ruled out by demonstrating that, of 44 control group participants with T cells responsive to 4 human common cold coronavirus peptides, only 1 was T-SPOT.COVID reactive.

Conclusion

The T-SPOT.COVID test performed well in detecting SARS-CoV-2-sensitized T-cells over many months

Introduction

Long-term protection from infectious agents, such as the SARS-CoV-2 virus, is mediated by T cells and antibody-mediated immunity of the adaptive immune system (

Sette and Crotty, 2021

). The T-SPOT.COVID test was developed to identify the presence of SARS-CoV-2-responsive T cells.

T cells contribute to the understanding of SARS-CoV-2 infections in many ways. T cells can identify past SARS-CoV-2 infections at a time when PCR tests would be negative and antibodies levels may be waning (

Dan et al., 2021

;

Gudbjartsson et al., 2020

;

Poland et al., 2020

). T cells can provide immune memory lasting for months (

Dan et al., 2021

) and perhaps years, as suggested by the discovery of T cells to the SARS-CoV-1 coronavirus 17 years after infection (

Le Bert et al., 2020

). T cells may act independently of antibodies to control a SARS-CoV-2 infection, as shown by the recovery of COVID-19 patients who lack detectable antibodies but have SARS-CoV-2-responsive T cells (

Gallais et al., 2021

;

Sekine et al., 2020

). T cells also show reactivity to numerous SARS-CoV-2 epitopes, so have the potential to protect against many SARS-CoV-2 variants (

Grifoni et al., 2020

;

Tarke et al., 2021

). T cell-based assays can probe the longevity of an immune response following a SARS-CoV-2 infection or vaccination (

Goletti et al., 2021

;

Liu et al., 2021

;

Reynolds et al., 2021

). These various roles suggest that a T cell assay can be a key contributor to SARS-CoV-2 investigations.

The T-SPOT.COVID test, an enzyme-linked immunospot (ELISpot) assay, identifies T cells in peripheral blood that release interferon-gamma (IFN-γ) in response to stimulation with SARS-CoV-2 peptides. The T-SPOT.COVID test builds on the T-SPOT platform (Oxford Immunotec) used worldwide for tuberculosis and cytomegalovirus testing and the research version, the T-SPOT Discovery SARS-CoV-2 test (

Liu et al., 2021

;

t.spot covid
t.spot covid
Wyllie et al., 2021

). The T-SPOT.COVID ELISpot methodology is performed in many laboratories and offers a standardized comparison of T cell immunity among participants. In addition, ELISpot assays normalize the number of peripheral blood mononuclear cells (PBMCs), thus maintaining test effectiveness in participants with lymphopenia, a commonly reported condition in many COVID-19 patients (

Altmann and Boyton, 2020

) and immunosuppressed people.

The objective of this study was to evaluate the ability of the T-SPOT.COVID test to detect T cell responses in participants with or without a history of SARS-CoV-2 infection and to compare the T-SPOT.COVID test results with anti-N immunoglobulin (Ig)G serology results in the first several months after infection.

Materials and Methods

2.1 Participant recruitment

Participants for this single-center, cross-sectional study were recruited from patients who had attended the outpatient Primacare medical center in Fall River, Massachusetts, USA, between November 30, 2020, and March 24, 2021, a time of high demand for COVID-19 testing. Among other healthcare services, Primacare provided COVID-19 testing to anyone wanting or required to be tested. The New England Center for Clinical Research (NECCR) invited participants to join the study if they had received a positive SARS-CoV-2 nucleic acid amplification test (NAAT) at Primacare or if NECCR deemed them to be at low risk of SARS-CoV-2 infection. As this study was run independently from the participants’ healthcare providers, clinical data such as chest x-rays and hospitalizations records were not obtained. Informed consent and study approval were obtained from the Advarra institutional review board by NECCR at Primacare.
Confirmed-infection group: A NAAT, which detects the presence of the SARS-CoV-2 virus, was used to identify people infected with SARS-CoV-2 at the time of testing (

Rai et al., 2021

). Participants in the confirmed-infection group were recruited from asymptomatic and symptomatic patients who had had a positive SARS-CoV-2 NAAT result within the past 9 months. The date of the first positive NAAT result was considered the date of diagnosis of SARS-CoV-2 infection. Blood was drawn for Abbott SARS-CoV-2 chemiluminescent microparticle immunoassay (CMIA) anti-N IgG serology and T-SPOT.COVID tests between 0 to 249 days after diagnosis.

The analysis of responses was divided into 3 time periods: 0 to 2 weeks after diagnosis (0 to 14 days); 2+ to 8 weeks after diagnosis (15 to 56 days); and 8+ weeks after diagnosis (57+ days).
Control group: Many SARS-CoV-2 studies use frozen pre-pandemic blood for control samples; however, the T-SPOT platform requires fresh blood to ensure consistent results. Therefore fresh blood was obtained from control group participants prospectively recruited from individuals with low risk of prior SARS-CoV-2 infection. Requirements for enrollment included no current or prior signs or symptoms of COVID-19, no known contact with a confirmed SARS-CoV-2-infected individual, no prior history of a positive SARS-CoV-2 NAAT, no SARS-CoV-2 vaccination, and no prior diagnosis with SARS-CoV-1 or Middle Eastern Respiratory Syndrome (MERS). In addition, the BIOHIT HealthCare SARS-CoV-2 lateral flow anti-N IgM/IgG serology test was performed at enrollment, and the 1 person with a positive BIOHIT result was not enrolled. Blood was drawn at enrollment for testing with T-SPOT.COVID and the Abbott CMIA anti-N IgG serology test and anyone with a positive serology result was excluded from the control group.

2.2 T-SPOT.COVID test

The T-SPOT.COVID test includes over 250 SARS-CoV-2 peptides (15-mer peptides overlapping by 11 amino acids) in 2 antigen peptide pools; one pool contains peptides from the spike S1 protein, including the receptor-binding domain, and the other contains peptides from the nucleocapsid protein.
Blood samples for the T-SPOT.COVID test were processed and analyzed according to the manufacturer’s instructions. Briefly, blood samples were drawn into lithium heparin tubes which were shipped overnight to Oxford Immunotec (Abingdon, UK) in temperature-controlled shipping boxes. Next, the T-Cell Xtend reagent (Oxford Immunotec) was added to the samples, and PBMCs were isolated by density gradient centrifugation, washed, counted, and 250 000 cells/well were plated into 4 wells of a 96-well plate.

COVID-19 variant pack
SB072 0.5
EUR 2325.81
Description: SARS-CoV-2 Spike (S) glycoprotein:Spike (S) glycoprotein corresponds to one of the leading targets for COVID-19 disease. Present on the surface of Sars-CoV-2 virus, Spike S protein in a class I fusion protein that allows the virus to enter host cells.Variants package available:Some proteins of Sars-CoV-2 are identified as leading targets for COVID-19 therapies. SB-PEPTIDE offers a special pack pre-made peptide libraries of Spike protein including peptides from COVID-19 variants: UK COVID-19 variant B.1.1.7, South Africa COVID-19 variant B.1.351, Brazil COVID-19 variant B.1.1.248. SB-PEPTIDE offers additional plate containing only peptides with Spike S protein mutation of COVID-19 B.1.1.7, B.1.3.5.1 and B.1.1.248 (cited below).Variants package and Spike (S) glycoprotein peptide library can be used for T-cell assays, immune monitoring, antigen specific T-cell stimulation, T-cell expansion and cellular immune response.

Moderna COVID-19 Vaccine
H7N7-319 1 vial
EUR 798.4
Description: Protein

SARS & COVID-19 coronavirus
3851 100 ug
EUR 330
Description: nucleoprotein

SARS & COVID-19 coronavirus
3861 100 ug
EUR 330
Description: nucleoprotein

SARS & COVID-19 coronavirus
3862 each
EUR 330
Description: nucleoprotein

SARS & COVID-19 coronavirus
3863 each
EUR 330
Description: nucleoprotein

SARS & COVID-19 coronavirus
3864 each
EUR 330
Description: nucleoprotein

COVID-19 Negative Control
MBS412771-05mL 0.5mL
EUR 100

COVID-19 Negative Control
MBS412771-5x05mL 5x0.5mL
EUR 305

Coronavirus COVID-19 Spike (COVID-19, SARS-CoV-2, 2019-nCoV) ELISA Kit
MBS7612502-10x96StripWells 10x96-Strip-Wells
EUR 3900

Coronavirus COVID-19 Spike (COVID-19, SARS-CoV-2, 2019-nCoV) ELISA Kit
MBS7612502-48StripWells 48-Strip-Wells
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Coronavirus COVID-19 Spike (COVID-19, SARS-CoV-2, 2019-nCoV) ELISA Kit
MBS7612502-5x96StripWells 5x96-Strip-Wells
EUR 2045

Coronavirus COVID-19 Spike (COVID-19, SARS-CoV-2, 2019-nCoV) ELISA Kit
MBS7612502-96StripWells 96-Strip-Wells
EUR 455

COVID-19 S Protein / (GFP)– (6His) VLP COVID-19 S Protein / (GFP)– (6His) VLP
VLP001 1x108 VP/ml x 200ul
EUR 455
Description: COVID-19 Spike protein (S) Virus Like Particle, packaged with GFP genomic material. Particles were concentrated and provided in PBS solution

Coronavirus (COVID-19) Antibody
MBS569951-1mg 1mg
EUR 655

Coronavirus (COVID-19) Antibody
MBS569951-5x1mg 5x1mg
EUR 2760

COVID-19 Nucleocapsid Protein
MBS669369-005mg 0.05mg
EUR 500

COVID-19 Nucleocapsid Protein
MBS669369-025mg 0.25mg
EUR 1010

COVID-19 Nucleocapsid Protein
MBS669369-5x025mg 5x0.25mg
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COVID-19 Nucleocapsid protein
30-2005 1 mg Ask for price

COVID-19 Nucleocapsid protein
30-2006 1 mg Ask for price

COVID-19 Nucleoprotein antibody
MBS5316694-1mg 1mg
EUR 360

COVID-19 Nucleoprotein antibody
MBS5316694-5x1mg 5x1mg
EUR 1480

COVID-19 Nucleoprotein antibody
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COVID-19 Nucleoprotein antibody
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STAT-NAT COVID-19 B
BSV-qPCR-12 24 tests
EUR 316
Description: STAT-NAT COVID-19 B

STAT-NAT COVID-19 HK
BSV-qPCR-13 24 tests
EUR 316
Description: STAT-NAT COVID-19 HK

COVID-19 Spike ELISA Kit
MBS7608267-10x96StripWells 10x96-Strip-Wells
EUR 3900

COVID-19 Spike ELISA Kit
MBS7608267-48StripWells 48-Strip-Wells
EUR 340

COVID-19 Spike ELISA Kit
MBS7608267-5x96StripWells 5x96-Strip-Wells
EUR 2045

COVID-19 Spike ELISA Kit
MBS7608267-96StripWells 96-Strip-Wells
EUR 455

STAT-NAT COVID-19 MULTI
BSV-qPCR-11 96 tests
EUR 758
Description: STAT-NAT COVID-19 MULTI

Diatheva COVID-19 PCR Kit
D-MBK0100 96 TEST
EUR 959.2

Covid 19 spike RBD
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AIRBORNE ALLERGY SCREEN KIT
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test for allergies airborne screen

Overview

During allergy skin tests, your skin is exposed to suspected allergy-causing substances (allergens) and is then observed for signs of an allergic reaction.

Along with your medical history, allergy tests may be able to confirm whether a particular substance you touch, breathe, or eat is causing symptoms.

 

Allergy skin tests are widely used to help diagnose allergic conditions, including:

  • Hay fever (allergic rhinitis)
  • Allergic asthma
  • Dermatitis (eczema)
  • Food allergies
  • Penicillin allergy
  • Bee venom allergy

Skin tests are generally safe for adults and children of all ages, including infants. In certain circumstances, though, skin tests aren’t recommended. Your doctor may advise against skin testing if you:

  • Have ever had a severe allergic reaction. You may be so sensitive to certain substances that even the tiny amounts used in skin tests could trigger a life-threatening reaction (anaphylaxis).
  • Take medications that could interfere with test results. These include antihistamines, many antidepressants and some heartburn medications. Your doctor may determine that it’s better for you to continue taking these medications than to temporarily discontinue them in preparation for a skin test.
  • Have certain skin conditions. If severe eczema or psoriasis affects large areas of skin on your arms and back — the usual testing sites — there may not be enough clear, uninvolved skin to do an effective test. Other skin conditions, such as dermatographism, can cause unreliable test results.

Blood tests (in vitro immunoglobulin E antibody tests) can be useful for those who shouldn’t or can’t undergo skin tests. Blood tests aren’t used for penicillin allergy.

In general, allergy skin tests are reliable for diagnosing allergies to airborne substances, such as pollen, pet dander and dust mites. Skin testing may help diagnose food allergies. But because food allergies can be complex, you may need additional tests or procedures.

Types of allergens

Allergens are substances that can cause an allergic reaction. There are three primary types of allergens:

  • Inhaled allergens affect the body when they come in contact with the lungs or membranes of the nostrils or throat. Pollen is the most common inhaled allergen.
  • Ingested allergens are present in certain foods, such as peanuts, soy, and seafood.
  • Contact allergens must come in contact with your skin to produce a reaction. An example of a reaction from a contact allergen is the rash and itching caused by poison ivy.

Allergy tests involve exposing you to a very small amount of a particular allergen and recording the reaction.

Insect sting allergy tests 

Why allergy testing is performed

Allergies affect more than 50 million people living in the USA, according to the American College of Allergy, Asthma, and Immunology. Inhaled allergens are by far the most common type. Seasonal allergies and hay fever, which is an allergic response to pollen, affect more than 40 million Americans.

The World Allergy Organization estimates that asthma is responsible for 250,000 deaths annually. These deaths can be avoided with proper allergy care, as asthma is considered an allergic disease process.

How allergy testing is performed

An allergy test may involve either a skin test or a blood test. You may have to go on an elimination diet if your doctor thinks you might have a food allergy.

AIRBORNE ALLERGY SCREEN KIT
AIRBORNE ALLERGY SCREEN KIT

Skin tests

Skin tests are used to identify numerous potential allergens. This includes airborne, food-related, and contact allergens. The three types of skin tests are scratch, intradermal, and patch tests.

Your doctor will typically try a scratch test first. During this test, an allergen is placed in liquid, then that liquid is placed on a section of your skin with a special tool that lightly punctures the allergen into the skin’s surface. You’ll be closely monitored to see how your skin reacts to the foreign substance. If there’s localized redness, swelling, elevation, or itchiness of the skin over the test site, you’re allergic to that specific allergen.

If the scratch test is inconclusive, your doctor may order an intradermal skin test. This test requires injecting a tiny amount of allergen into the dermis layer of your skin. Again, your doctor will monitor your reaction.

Another form of skin test is the patch test (T.R.U.E. TESTTrusted Source). This involves using adhesive patches loaded with suspected allergens and placing these patches on your skin. The patches will remain on your body after you leave your doctor’s office. The patches are then reviewed at 48 hours after application and again at 72 to 96 hours after application.

Blood tests

If there’s a chance you’ll have a severe allergic reaction to a skin test, your doctor may call for a blood test. The blood is tested in a laboratory for the presence of antibodies that fight specific allergens. This test, called ImmunoCAP, is very successful in detecting IgE antibodies to major allergens.

Elimination diet

An elimination diet may help your doctor determine which foods are causing you to have an allergic reaction. It entails removing certain foods from your diet and later adding them back in. Your reactions will help determine which foods cause problems.

 

 

Leptospira IgG / IgM Rapid Test Kit
abx092260-1ml 1 ml
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abx092260-200l 200 µl
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Canine Leptospirosis Virus Antibody Rapid Test Kit
RPS99137
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414 each
EUR 270
Description: LipL41

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411 each
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412 each
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413 each
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EUR 235
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Rat leptospira antibody IgG(Leptospira-Igg) Elisa Kit
EK720973 96 Wells
EUR 0.62

Rat leptospira antibody IgG(Leptospira-Igg) ELISA Kit
SL1029Ra -
EUR 498

Mouse Leptospira antibody IgG(Leptospira-IgG) ELISA Kit
SL0345Mo -
EUR 528

Bovine Leptospira antibody IgG (Leptospira AB IgG) ELISA Kit
YLA0363BO-48T 48T Ask for price

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DELEPVT0660 96
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Leptospira IgG
MBS191665-3mL 3mL
EUR 230

Leptospira IgG
MBS191665-5x3mL 5x3mL
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MBS191666-3mL 3mL
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PCR-VH089-PCRVH08948D PCR-VH089-48D
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Leptospira biflexa
MBS324029-1mL 1mL
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Leptospira biflexa
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Leptospira biflexa
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Leptospira biflexa
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Leptospira Antibody
MBS568468-05mL 0.5mL
EUR 300

Leptospira Antibody
MBS568468-5x05mL 5x0.5mL
EUR 1160

Leptospira antibody
MBS832159-05mg 0.5mg
EUR 585
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